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目的本研究旨在检测HPV16E7基因在新疆南部维吾尔妇女宫颈癌组织中的突变。方法从35份新疆南部地区维吾尔族妇女宫颈癌活检组织标本中提取组织DNA,作为模板,PCR扩增HPV16 E7全长基因,PCR产物直接测序或克隆后测序,分析新疆维吾尔族妇女宫颈癌组织HPV16E7基因的突变。结果PCR检测结果宫颈癌组织中HPV16E7阳性率为82.86%(29/35);27个E7分离片段的测序和序列分析表明,26个分离株E7基因与原型相同,1个分离株E7基因核苷酸发生2处突变,即647位(在HPV16基因组中的位置)的A→G,氨基酸由Asn变异为Ser;845位的T→C,氨基酸不变。结论新疆南部地区维吾尔妇女宫颈癌患者感染的HPV16中存在E7基因的变异株,该变异株可能是由人口流动传入。
Objective This study was designed to detect the mutation of HPV16E7 gene in Uighur women with cervical cancer in southern Xinjiang. Methods Tissue DNA was extracted from 35 samples of uterine cervix cancer biopsy specimens of southern Uygur Xinjiang women. The full length HPV16 E7 gene was amplified by PCR. The PCR products were directly sequenced or sequenced. The expression of HPV16 E7 Gene mutation. Results The positive rate of HPV16E7 in cervical cancer tissues was 82.86% (29/35) by PCR. The sequencing and sequence analysis of 27 E7 isolates showed that the E7 gene of 26 isolates was the same as the original one, and one isolate of E7 gene nucleoside The acid undergoes two mutations, namely, A → G at position 647 (position in the HPV16 genome), the amino acid changes from Asn to Ser, and the T → C at position 845 does not change. Conclusion There is a variant of E7 gene in HPV16 of Uighur women with cervical cancer in southern Xinjiang, which may be introduced by population movement.