在反义实验中成功的关键是选择合适的靶位点。但是 ,由于RNA折叠形成二级结构 ,会使大多数互补的核酸不能接近并形成分子间的碱基配对 ,因此这种选择非常困难。通常采用的方法 ,如“序列步移”或计算机辅助设计 ,并未获得显著的成功。为此 ,本文总结了几个经验选择方法 ,特别是基于转录物的RNaseH图谱和寡核苷酸芯片扫描的方法。 The key to success in antisense experiments is to select the appropriate target site. However, this choice is very difficult because the RNA folds to form secondary structures, rendering most complementary nucleic acids inaccessible and forming intermolecular base-pairing. Commonly used methods, such as “sequence walk” or computer-aided design, did not achieve significant success. To this end, this article summarizes several empirical selection methods, in particular transcript-based RNaseH mapping and oligonucleotide chip scanning methods.