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目的 探讨小鼠骨髓间充质干细胞经脉冲电磁场辐射不同时间后其细胞增殖水平的变化。方法 用密度梯度离心法分离小鼠骨髓间充质干细胞 ,再经贴壁筛选法筛选 ,对生长良好的第 3代小鼠骨髓间充质干细胞进行不同时间的脉冲电磁场辐射 (频率 50Hz、正弦波形、不同强度 ) ,用MTT法、流式细胞仪法测定细胞生长增殖水平及细胞周期变化。结果 小鼠骨髓间充质干细胞经脉冲电磁场辐射 3d(每天 2次 ,每次 3 0min)后 ,各实验组细胞增殖水平均有明显提高 ,差异有显著性意义 (P <0 .0 5)。采用流式细胞仪测定细胞周期 ,发现各实验组 (S +G2 /M )期细胞数量较对照组均有不同程度的增长 ,差异有显著性意义 (P <0 .0 5)。各组均未发现DNA倍体异常细胞。结论 小鼠骨髓间充质干细胞经频率为 50Hz、正弦波形、强度分别为 4mT ,3mT ,2mT ,1mT的脉冲电磁场辐射 3d(每天 2次 ,每次 3 0min ,间隔 12h)后 ,能明显促进该细胞体外培养的增殖水平
Objective To investigate the changes of cell proliferation in mouse bone marrow mesenchymal stem cells (BMSCs) irradiated by pulsed electromagnetic fields at different time. Methods Mouse bone marrow mesenchymal stem cells were isolated by density gradient centrifugation and then screened by adherent screening. The third generation of mouse bone marrow mesenchymal stem cells were irradiated with pulsed electromagnetic field (frequency 50Hz, sinusoidal waveform , Different intensity), MTT assay, flow cytometry determination of cell growth and proliferation levels and cell cycle changes. Results The proliferation of mouse bone marrow mesenchymal stem cells (BMSCs) in each experimental group were significantly increased after being irradiated by pulsed electromagnetic field for 3 days (twice a day, 30 min each) (P <0.05). Flow cytometry was used to determine the cell cycle. The number of cells in each experimental group (S + G2 / M) was significantly higher than that in the control group (P <0.05). No DNA ploidy cells were found in all groups. Conclusions The mouse bone marrow mesenchymal stem cells (BMSCs) can radiate 3d (2 times a day, 30 minutes each time, 12 hours apart) with frequency of 50Hz, sinusoidal waveform and intensity of 4mT, 3mT, 2mT and 1mT respectively. Cell proliferation in vitro culture