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目的探讨甲型H1N1流感患者Th17细胞下降的可能机制。方法 40名健康人、53名单纯甲型H1N1流感患者、16名并发肺炎甲型H1N1流感患者入选本研究,以ELISA方法测定三组人群血浆TGF-β、IL-6、IL-1-β、IL-22、IL-23、IFN-γ水平,实时定量RT-PCR方法测定三组人群PBMC中RORγt、STAT3、SOCS3表达水平。统计学方法采用单因素方差分析,统计软件采用Graphpad Prism 4.0。结果甲型H1N1流感患者血浆TGF-β水平较健康人显著下降,并发肺炎甲型H1N1流感患者血浆TGF-β水平进一步显著下降(F=32.42,P<0.0001)。单纯甲型H1N1流感及并发肺炎甲型H1N1流感患者PBMC中STAT3表达显著低于健康人(F=4.599,P=0.0176)。结论甲型H1N1流感病毒可能通过降低血中TGF-β水平抑制核转录因子STAT3的表达,从而抑制Th17细胞的分化。
Objective To investigate the possible mechanism of Th17 cell decline in patients with influenza A (H1N1). Methods Forty healthy subjects, 53 patients with simple H1N1 influenza and 16 patients with influenza A (H1N1) pneumonia were enrolled in this study. The levels of plasma TGF-β, IL-6, IL-1β, The levels of RORγt, STAT3 and SOCS3 in peripheral blood mononuclear cells of three groups were measured by real-time quantitative RT-PCR. The levels of IL-22, IL-23 and IFN- Statistical methods using one-way analysis of variance, statistical software using Graphpad Prism 4.0. Results The plasma level of TGF-β in patients with type A H1N1 influenza was significantly lower than that in healthy people. The level of TGF-β in patients with influenza A (H1N1) pneumonia was further significantly decreased (F = 32.42, P <0.0001). STAT3 expression in PBMC of patients with influenza A (H1N1) and influenza A (H1N1) complicated with pneumonia was significantly lower than that of healthy people (F = 4.599, P = 0.0176). Conclusion Influenza A (H1N1) virus may inhibit the differentiation of Th17 cells by decreasing the level of TGF-β in blood and inhibiting the expression of nuclear factor STAT3.