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AIM:Helicobacter pylori(Hpylori)has been linked to chronicgastritis,peptic ulcer,gastric cancer and MALT-lymphoma.The link of genotypes of Hpylorito gastric cancer remainscontroversial.The aim of this study was to investigate theHpylori vacA alleles,cagA and iceA in patients with gastriccancer in Taiwan.METHODS:Patients with gastric cancer,peptic ulcer andchronic gastritis were enrolled in this study.We obtainedbiopsy specimens from the stomach at least 2 cm awayfrom the tumor margin in patients with gastric cancer,andfrom the antrum of stomach in patients with peptic ulceror chronic gastritis.DNA extraction and polymerase chainreaction were used to detect the presence or absence ofcagA and to assess the polymorphism of vacA and iceA.RESULTS:A total of 168 patients(gastric ulcer:77,duodenalulcer:66,and chronic gastritis:25)were found to havepositive PCR results of the biopsy specimens from patientswith peptic ulcer and chronic gastritis.We found positivecagA(139/168,83%),m2(84/168,50%)and iceA1(125/168,74%)strains in the majority of patients.In patients withgastric cancer,the vacA sla and slc subtypes were lesscommonly found than those in non-cancer patients(35/66 vs127/168,P=0.0001 for sla and 13/66 vs93/168,P<0.0001for slc).In the middle region,the ml T strain in patientswith gastric cancer was more than that of non-cancer patients(23/66 vs33/168,P=0.02).CONCLUSION:In Taiwan,Hpyloriwith positive vacA sla,cagA and iceAl strains are found in the majority of patientswith gastric cancer or non-cancer patients.In patients withgastric cancer,the vacA sla and slc subtypes are lessand mlT is more than in patients with peptic ulcer andchronic gastritis.
AIM: Helicobacter pylori (Hpylori) has been linked to chronic gastritis, peptic ulcer, gastric cancer and MALT-lymphoma. The link of genotypes of Hpylorito gastric cancer remains controversial. The AIM of this study was to investigate the H. pylori vacA alleles, cagA and iceA in patients with gastriccancer in Taiwan. METHODS: Patients with gastric cancer, peptic ulcer andchronic gastritis were enrolled in this study. We obtainedbiopsy specimens from the stomach at least 2 cm awayfrom the tumor margin in patients with gastric cancer, andfrom the antrum of stomach in patients with peptic ulceror chronic gastritis. DNA extraction and polymerase chain reaction was used to detect the presence or absence of cagA and to evaluate the polymorphism of vacA and iceA.RESULTS: A total of 168 patients (gastric ulcer: 77, duodenalulcer: 66, and chronic gastritis: 25) were found to have positive PCR results of the biopsy specimens from patients with peptic ulcer and chronic gastritis. We found positive cagA (139 / 168,83%), m2 (84/168, 50%) and iceA1 (125/168, 74%) in the majority of patients. In patients with gastric cancer, the vacA sla and slc subtypes were less commonly found than those in non-cancer patients (35/66 vs 127/168, P = 0.0001 for sla and 13/66 vs 93/168, P <0.0001 for slc). In the middle region, the ml T strain in patients with gastric cancer was more than that of non-cancer patients (23/66 vs 33/168, P = 0.02) .CONCLUSION: In Taiwan, Hpylori with positive vacA sla, cagA and iceAl strains are found in the majority of patients with gastric cancer or non-cancer patients. In patients with gastric cancer, the vacA sla and slc subtypes are less and mlT is more than in patients with peptic ulcer andchronic gastritis.