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建立了人降结肠高分化管状腺癌裸鼠移植瘤,历时两年余已传19代。研究表明,移植瘤的组织形态各代之间基本相同,呈高分化管状腺癌。腺管上皮细胞呈柱状,核梭形或不规则形,核仁明显,核位于细胞基底部呈极性排列;腺腔侧可见胞浆带。少数癌巢上皮细胞排列极性消失。在肿瘤相关抗原表达方面,CEA抗原呈弥漫性胞浆阳牲,人大肠癌抗原(MC_3)呈胞浆和腺腔缘阳性,腺管旁浸润小巢的阳性程度明显高于腺管。对9代移植瘤进行电镜观察,各移植瘤癌细胞超微形态呈现异型性,但腺腔缘可见微绒毛。提示我们建立的人降结肠高分化管状腺癌裸鼠移植瘤适合作为人大肠管状腺癌生物学行为和治疗研究的较好模型,MC_3抗原表达强弱可能同癌细胞的增强和侵袭力有关,以MC_3全胞浆阳性的癌细胞增殖和侵袭力更强。
A transplantable tumor with well-differentiated tubular adenocarcinoma of the human descending colon was established and it has been transmitted for 19 generations in more than two years. Studies have shown that the histological morphology of the transplanted tumors is essentially the same between generations, showing highly differentiated tubular adenocarcinoma. The glandular epithelial cells were columnar, spindle-like or irregularly shaped, and the nucleolus were prominent. The nucleus was located at the base of the cells in a polar arrangement; the cytoplasmic band was seen on the side of the glandular lumen. A few cancer nest epithelial cells disappeared in polarity. In the expression of tumor-associated antigens, CEA antigen was diffuse cytoplasmic positive animal, human colorectal cancer antigen (MC_3) was positive in cytoplasm and glandular margins, and the positive degree of infiltration of small nests adjacent to the glandular ducts was significantly higher than that of the ducts. Electron microscopy was performed on the 9th generation of transplanted tumors. The ultrastructure of tumor cells in each transplanted tumor showed abnormality, but microvilli were seen on the margin of the glandular cavity. It is suggested that the human transplantable tumor with well-differentiated tubular adenocarcinoma of the descending colon is suitable as a good model for biological behavior and therapeutic research of human large intestinal tubular adenocarcinoma. The expression of MC3 antigen may be related to the enhancement and invasiveness of cancer cells. Cancer cells positive for MC_3 cytoplasm were more proliferating and invasive.