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目的分离酒精性肝硬化病人的高表达基因 E251,并研究它的表达.方法与结果从我们以前在酒精性肝硬化大鼠中分离到的一个新基因 E251中,设计一对引物用逆转录 PCR 法从酒精性肝硬化病人的肝组织中分离到人肝 E251基因,DNA 序列对比证实与大鼠肝的 E251为同源基因,有大于95%的同源性.它的 mRNA 表达在酒精性肝硬化病人中比正常人高约2倍,说明在酒精刺激下,E251基因的表达增强.人肝 E251约0.95Kb 大小,含有 AATAAA的特别序列,并含有两个蛋白激酶 C 磷酸化激活位点.结论 1.存在与大鼠 E251同源的人类 E251基因;2.在酒精性肝硬化病人中,E251的表达增强.
OBJECTIVE: To isolate the highly expressed gene E251 from patients with alcoholic liver cirrhosis and to study its expression.Methods and Results From a new gene E251 previously isolated from alcoholic cirrhotic rats, a pair of primers was designed by reverse transcription PCR Human liver E251 gene was isolated from the liver tissue of patients with alcoholic cirrhosis and the DNA sequence comparison confirmed that it has homology of more than 95% with that of rat liver E251.Its mRNA expression in alcoholic liver Sclerosis patients about 2 times higher than normal, indicating that under the stimulation of alcohol, E251 gene expression enhanced human liver E251 about 0.95Kb size, containing AATAAA special sequence, and contains two protein kinase C phosphorylation activation sites. Conclusions 1. There is a human E251 gene homologous to rat E251; 2. In patients with alcoholic cirrhosis, E251 expression is enhanced.