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目的：探讨卵巢肿瘤组织中ｎｍ２３－Ｈ１的表达和突变与其浸润转移的关系。方法：采用免疫组织化学，反转录多聚酶链反应（ＲＴ－ＰＣＲ）和多聚酶链反应单链构象多态技术（ＰＣＲＳＳＣＰ）方法检测４１例卵巢癌，２０例卵巢良性肿瘤和２１例正常卵巢组织ｎｍ２３－Ｈ１的表达和突变。结果：（１）卵巢癌组织中ｎｍ２３－Ｈ１阳性表达率明显高于良性卵巢肿瘤及正常对照（Ｐ＜００１）；（２）卵巢癌灶ｎｍ２３－Ｈ１的阳性表达及扩增明显高于转移灶（Ｐ＜００１）；（３）Ⅰ～Ⅱ期患者癌组织中ｎｍ２３－Ｈ１的阳性表达率明显高于Ⅲ～Ⅳ期者（Ｐ＜００１）；（４）ｎｍ２３Ｈ的阳性表达和扩增与病理分级及病理分类无显著差别（Ｐ＞００５）；（５）ｎｍ２３Ｈ１阳性表达的患者近期疗效比阴性者好（Ｐ＜００５）。（６）卵巢癌组织中ｎｍ２３－Ｈ１的突变率为７３％且均发生在晚期患者，而良性肿瘤和正常对照未发现有突变。结论：ｎｍ２３－Ｈ１阳性表达与卵巢癌的浸润转移有关，对它的检测有助于临床上判断预后。
Objective: To investigate the relationship between the expression of nm23-H1 and its infiltration and metastasis in ovarian tumors. Methods: Immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR) and polymerase chain reaction-single strand conformation polymorphism (PCRSSCP) were used to detect 41 cases of ovarian cancer, 20 cases of benign ovarian tumors and 21 cases of benign ovarian tumors Expression and mutation of nm23-H1 in normal ovarian tissue. Results: (1) The positive rate of nm23-H1 in ovarian cancer tissues was significantly higher than that in benign ovarian tumors and normal controls (P <001). (2) The positive expression and amplification of nm23-H1 in ovarian cancer was significantly higher than that in benign ovarian tumors (3) The positive expression rates of nm23-H1 in stage Ⅰ-Ⅱ cancer tissues were significantly higher than those in stage Ⅲ-Ⅳ (P <001); (4) nm23H (P> 0.05). (5) The positive rate of positive expression of nm23 H1 was significantly lower than that of negative (P <005). (6) The mutation rate of nm23-H1 in ovarian cancer tissues was 7.3%, both of which were in advanced stage, while no mutation was found in benign tumors and normal controls. Conclusion: The positive expression of nm23-H1 is correlated with the invasion and metastasis of ovarian cancer. The detection of nm23-H1 is helpful to judge the prognosis clinically.