目的分析乳腺癌干细胞(BCSCs)亚群比例的改变与芳香化酶抑制剂(AIs)耐药之间的相关性。方法采用流式细胞术检测对AIs耐药的人源性乳腺癌细胞株MCF-7(MCF-7/AI)及野生型人源性乳腺癌细胞株MCF-7中CD44+CD24-细胞的数量,比较MCF-7/AI和MCF-7中CD44+CD24-细胞的比例。结果 MCF-7/AI和MCF-7中CD44+CD24-细胞的平均比例分别为(13.15±5.50)%和(0.39±0.22)%(P<0.05)。结论 MCF-7/AI中BCSCs亚群的比例显著高于MCF-7,提示AIs耐药和BCSCs之间可能存在着一定的相关性,BCSCs亚群比例的改变可能是AIs耐药的重要标志之一。 Objective To analyze the correlation between the changes of the proportion of subpopulations of breast cancer stem cells (BCSCs) and the resistance of aromatase inhibitors (AIs). Methods Flow cytometry was used to detect the number of CD44 + CD24- cells in human breast cancer cell line MCF-7 (MCF-7 / AI) and wild-type human breast cancer cell line MCF-7 , The proportion of CD44 + CD24- cells in MCF-7 / AI and MCF-7 was compared. Results The average proportion of CD44 + CD24- cells in MCF-7 / AI and MCF-7 was (13.15 ± 5.50)% and (0.39 ± 0.22)%, respectively (P <0.05). Conclusions The proportion of BCSCs in MCF-7 / AI is significantly higher than that in MCF-7, which suggests that there may be some correlation between AIs resistance and BCSCs. The change of BCSCs subpopulation may be an important marker of AIs resistance one.