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目的:探讨中国人白细胞介素-10基因启动子单核苷酸多态性与乙型肝炎病毒感染之后临床发展之间的关系.方法:分别采用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)和聚合酶链反应-序列特异性引物扩增法(PCR-SSP)检测478例慢性乙型肝炎患者,223例乙肝病毒携带者及267例自限性感染者基因组DNAIL-10基因启动子区域3个多态位点-592、-819、-1082的基因多态性,并进行相关性分析.结果:IL-10-1082A等位基因及AA基因型在慢性乙型肝炎患者组的频率明显高于自限性感染者组和乙肝病毒携带者组(A:χ2=37.72,P=0.000;χ2=45.23,P=0.000;AA:χ2=20.53,P=0.000;χ2=19.14,P=0.000).IL-10-819T等位基因及TT基因型在慢性乙型肝炎患者组的频率也高于自限性感染者组和乙肝病毒携带者组(T:χ2=10.5,P<0.001;χ2=17.38,P<0.001;TT:χ2=8.76,P=0.003;χ2=5.656,P=0.017).IL-10-592C/A等位基因频率和AA/CC/AC基因型在三组的分布没有统计学意义(P>0.05).结论:IL-10基因启动子多态性与乙肝病毒感染后临床发展过程可能相关.
OBJECTIVE: To investigate the relationship between the single nucleotide polymorphism of interleukin-10 gene promoter in Chinese and the clinical development of hepatitis B virus infection.Methods: Polymerase chain reaction-restriction fragment length polymorphism PCR-RFLP and PCR-SSP were used to detect genomic DNAIL in 478 patients with chronic hepatitis B, 223 patients with hepatitis B virus and 267 patients with self-limited infection -10 gene promoter region of the three polymorphisms -592, -819, -1082 gene polymorphism, and correlation analysis.Results: IL-10-1082A allele and AA genotype in chronic type B The frequency of hepatitis patients was significantly higher than that of self-limited HIV and HBV carriers (A: χ2 = 37.72, P = 0.000; χ2 = 45.23, P = 0.000; AA: χ2 = 20.53, P = 0.000; = 19.14, P = 0.000). The frequencies of IL-10-819T allele and TT genotype in patients with chronic hepatitis B were also higher than those in self-limited HIV and HBV carriers (T: χ2 = 10.5 , P <0.001; χ2 = 17.38, P <0.001; TT: χ2 = 8.76, P = 0.003; χ2 = 5.656, P = 0.017). The frequencies of IL-10-592C / A allele and AA / CC / Type distribution in the three groups did not Considered significant (P> 0.05) Conclusion:. IL-10 promoter polymorphism may be associated with the clinical development of gene promoter of hepatitis B virus infection.