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目的:研究慈济化癌保生汤(CHBT)对H22肝癌化疗小鼠外周血细胞以及脾脏造血生长因子红细胞生成素(EPO)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)以及造血功能的作用。方法:将肝癌H22细胞悬液(2×107/m L)接种于Kunming种小鼠右前腋皮下,约7d后,全部形成移植瘤,环磷酰胺(CTX)按200mg/kg(0.2m L/10g)腹腔注射,建立H22肝癌小鼠化疗模型。随机分为模型组,CTX(0.033g/kg)组,CHBT高、中、低剂量(117、58.5、29.25g/kg)组,连续给药10d,采用全自动血细胞分析仪检测外周血红细胞、白细胞、血小板和血红蛋白,运用酶联免疫吸附测定法检测脾组织EPO和GM-CSF含量。结果:与模型组和CTX组比较,CHBT高、中、低剂量组红细胞、白细胞、血小板数量明显升高(P<0.05,P<0.01);CHBT高、中剂量组血红蛋白升高(P<0.05,P<0.01);CHBT各剂量组EPO、GM-CSF含量升高(P<0.01,P<0.05)。结论:慈济化癌保生汤能促进化疗后EPO和GM-CSF的生成并增强活性,刺激血细胞的产生,维持外周血象稳定性。
Objective: To study the effects of TCHI on the peripheral blood cells of H22 hepatocarcinoma mice and the effects of hematopoietic growth factor erythropoietin (EPO), granulocyte-macrophage colony stimulating factor (GM-CSF) and hematopoietic function effect. Methods: The H22 cell suspension (2 × 107 / m L) was inoculated into the right forearm of Kunming mice. After about 7 days, all the tumor cells were transplanted. Cyclophosphamide (CTX) 10g) intraperitoneal injection, the establishment of H22 hepatocellular carcinoma mouse model of chemotherapy. The rats were randomly divided into model group, CTX (0.033g / kg) group, high, medium and low dose of CHBT (117,58.5,29.25g / kg) group, continuous administration of 10d, using automated hematology analyzer detection of peripheral blood red blood cells, White blood cells, platelets and hemoglobin, and the content of EPO and GM-CSF in spleen tissue were detected by enzyme-linked immunosorbent assay. Results: Compared with the model group and the CTX group, the numbers of erythrocytes, leukocytes and platelets in CHBT high, medium and low dose groups were significantly increased (P <0.05, P <0.01) , P <0.01). The contents of EPO and GM-CSF in CHBT groups increased (P <0.01, P <0.05). Conclusion: TCDC can promote the formation of EPO and GM-CSF after chemotherapy, enhance the activity, stimulate the production of blood cells and maintain the stability of peripheral blood.