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目的研究乙型肝炎病毒血清前S1抗原、HBV-DNA与其他乙型肝炎血清标志物HBs-Ag、HBeAg的相关性。方法采用实时聚合酶链反应(Real-Time PCR)技术检测血清中HBV-DNA的含量,用酶联免疫法(ELISA)检测PreS1Ag、HBsAg和HBeAg。采用SPSS 13.0统计软件进行分析,成组设计资料,率比较采用配对的χ2检验,结果的关联性采用独立性的χ2检验。结果以HBV-DNA<1.000E+03Copy/ml为阴性组;HBV-DNA≥1.000E+03Copy/ml为阳性组。600例HBsAg阳性的乙型肝炎患者中241例HBV-DNA阳性,283例Pre-S1阳性。其中241例HBV-DNA阳性患者中,Pre-S1阳性222例,阳性率为92.12%。显著高于HBV-DNA阴性组的Pre-S1阳性率(16.99%)。经配对的差异性检验P=0.000(确切概率)。经独立性检验,χ2=326.573,P=0.000。241例HBsAg、HBV-DNA阳性组,HBeAg阳性191例,Pre-S1阳性222例,其中191例HBeAg阳性患者中,Pre-S1Ag阳性185例,阳性率为96.86%。显著高于HBeAg阴性组的Pre-S1Ag阳性率(61.67%)。经配对的差异性检验P=0.000(确切概率)。经独立性检验,χ2=28.511,P=0.000。结论 Pre-S1Ag与HBV-DNA阳性高度相关,有好的一致性和互补性,较HBeAg更敏感。可作为乙肝病毒存在和复制的可靠标志,是反映HBV是否具有传染性的观察指标。
Objective To study the relationship between serum pre-S1 antigen and HBV-DNA of hepatitis B virus and HBsAg and HBeAg in other hepatitis B patients. Methods Real-time PCR was used to detect the content of HBV-DNA in serum and PreS1Ag, HBsAg and HBeAg were detected by enzyme-linked immunosorbent assay (ELISA). Using SPSS 13.0 statistical software for analysis, group design information, the rate of the use of paired χ2 test, the correlation of the results using independent χ2 test. Results HBV-DNA <1.000E + 03Copy / ml as negative group; HBV-DNA≥1.000E + 03Copy / ml as positive group. Of the 600 HBsAg-positive hepatitis B patients, 241 were HBV-DNA positive and 283 were Pre-S1 positive. Among 241 cases of HBV-DNA positive patients, Pre-S1 positive 222 cases, the positive rate was 92.12%. Which was significantly higher than that of HBV-DNA negative group (16.99%). Paired difference test P = 0.000 (exact probability). There were 191 cases of positive HBeAg and 222 cases of Pre-S1 positive in HBsAg and HBV-DNA positive group, of which 185 cases were pre-S1Ag positive in 191 cases of HBeAg-positive patients, The positive rate was 96.86%. The positive rate of Pre-S1Ag in HBeAg-negative group (61.67%) was significantly higher than that in HBeAg-negative group. Paired difference test P = 0.000 (exact probability). After independence test, χ2 = 28.511, P = 0.000. Conclusion Pre-S1Ag is highly correlated with HBV-DNA positive and has good consistency and complementarity, which is more sensitive than HBeAg. Can be used as a reliable indicator of the presence and replication of hepatitis B virus is an indicator of whether HBV is contagious.