Detailed radiolarian biostratigraphy in the Plio-Pleistocene was analyzed by using samples from IODP Site U1340 that was drilled to a core depth of 604 m in the southern Bering Sea.A total of 227 species belonging to 102 genera were identified.Based on the distributions of the radiolarian index species at Site U1340,five radiolarian zones since the Pliocene were established in the southern Bering Sea for the first time,and 25 radiolarian bioevents were recognized.Their ages were estimated on the basis of the age-depth plot that was constructed by the synthetical datum of the effective biostratigraphic and magnetostratigraphic events.The radiolarian zones at Site U1340 were systematically compared with those in its adjacent regions since the late Early Pliocene,which further improved and interpreted the biostratigraphic datum as well as their correlations in the middle-high latitude of the North Pacific.In addition,the comparative results of radiolarian zones show that Botryostrobus aquilonaris Zone emended in this paper is equivalent to the upper part of the same zone defined by Hays,1970,and Druppatractus irregularis-Dorydruppa bensoni Zone as well as Spongodiscus sp.Zone,newly proposed in this paper,are well correlated with Cycladophora sakaii Zone and Stylatractus universus Zone in the subarctic North Pacific,respectively. Detailed radiolarian biostratigraphy in the Plio-Pleistocene was analyzed by using samples from IODP Site U1340 that was drilled to a core depth of 604 m in the southern Bering Sea. A total of 227 species belongs to 102 genera were identified. Based on the distributions of the radiolarian index species at Site U1340, five radiolarian zones since the Pliocene were established in the southern Bering Sea for the first time, and 25 radiolarian bioevents were recognized. The ages were estimated on the basis of the age-depth plot that was constructed by the synthetical datum of the effective biostratigraphic and magnetostratigraphic events. the radiolarian zones at Site U1340 were systematically compared with those in early adjacent to livale, which further improved and interpreted the biostratigraphic datum as well as their correlations in the middle-high latitude of the North Pacific.In addition, the comparative results of radiolarian zones show that Botryostrobus aqu ilonaris Zone emended in this paper is equivalent to the upper part of the same zone defined by Hays, 1970, and Druppatractus irregularis-Dorydruppa bensoni Zone as well as Spongodiscus sp. Zone, newly proposed in this paper, are well correlated with Cycladophora sakaii Zone and Stylactus universus Zone in the subarctic North Pacific, respectively.