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镧系元素(Lns)中,镧(La)和钆(Gd)在临床诊断和治疗中发挥着重要作用,二者在临床中的应用会导致La和Gd蓄积于骨骼内。La和Gd物理化学性质相似,当二者应用于肾功能不全患者时,相似的性质是否会对骨代谢造成相似的生物学影响,正在引起研究人员关注。本文比较了在高磷条件下LaCl_3和Gd Cl_3对大鼠骨髓间充质干细胞(BMSCs)分化的作用,并探讨其可能机理。结果表明,在高磷条件下,LaCl_3高浓度抑制BMSCs分化,低浓度能够促进分化相关基因表达;而Gd Cl_3促进细胞分化,进一步研究发现,培养过程中生成的含Lns的钙磷酸盐沉淀参与了在本实验中观察到的La和Gd对细胞分化的不同作用。La和Gd在原子序数上存在微小差异,致使Gd对沉淀生成的促进作用更强;含Gd的钙磷酸盐颗粒吸附蛋白量少于含La的钙磷酸盐颗粒,导致含Gd的颗粒粒径较大。总之,Gd与La参与的颗粒理化性质上的不同部分导致了本实验中LaCl_3和Gd Cl_3对细胞分化作用的不同。
Among lanthanides (Lns), lanthanum (La) and gadolinium (Gd) play an important role in the clinical diagnosis and treatment, and their application in clinic leads to the accumulation of La and Gd in bone. The physical and chemical properties of La and Gd are similar. Whether the similar properties exert a similar biological effect on bone metabolism when they are used in patients with renal insufficiency is a cause of concern to researchers. This article compared the effects of LaCl 3 and Gd Cl 3 on the differentiation of rat bone marrow mesenchymal stem cells (BMSCs) under high phosphorus conditions and explored the possible mechanism. The results showed that high concentration of LaCl_3 inhibited the differentiation of BMSCs at low concentration and promoted the expression of differentiation-related genes at high concentration, while GdCl_3 promoted cell differentiation. Further studies showed that calcium phosphate precipitate containing Lns was involved in the culture The different effects of La and Gd on cell differentiation were observed in this experiment. La and Gd have slight difference in atomic number, which makes Gd promote the precipitation more strongly. The amount of Gd-containing calcium phosphate particles adsorbed protein is less than that of La-containing calcium phosphate particles, resulting in the particle size of Gd containing particles Big. In conclusion, the different parts of the physico-chemical properties of the particles involved in Gd and La led to differences in cell differentiation between LaCl 3 and Gd Cl 3 in this experiment.