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目的观察高脂血症大鼠主动脉脂联素受体1/2(AdipoR1/2)mRNA表达的改变以及吡格列酮干预效果。方法 SD大鼠随机分为正常饮食组(NC组)和高脂饮食组(HF组);HF组造模成功后,随机分为高脂组(HL组)和吡格列酮治疗组(Pio组),检测血脂、主动脉病理,以ELISA法检测血清脂联素水平,以实时荧光定量PCR法检测主动脉脂联素受体mRNA的表达。结果高脂饲料喂养12周,HF组TG、TC及LDL-C的水平明显升高(P<0.01)。给药4周后,与HL组比较,Pio组TG、TC明显降低(P<0.01),血清脂联素明显升高(P<0.05);与NC比较,HL组主动脉脂联素受体1/2mRNA表达明显下降(P<0.05);Pio组主动脉脂联素受体1/2mRNA表达明显升高(P<0.05或P<0.01)。结论脂联素受体表达下降可能介入高脂血症血管损伤,吡格列酮具有改善高脂血症血管损伤及抗动脉粥样硬化作用,该作用可能与提高主动脉血管脂联素受体表达有关。
Objective To observe the changes of AdipoR1 / 2 mRNA expression in aorta and the effect of pioglitazone intervention in hyperlipidemic rats. Methods SD rats were randomly divided into normal diet group (NC group) and high fat diet group (HF group). After HF model was successfully established, the rats were randomly divided into hyperlipidemia group (HL group) and pioglitazone treatment group (Pio group) Serum adiponectin level was detected by ELISA and the expression of adiponectin receptor mRNA in aorta was detected by real-time fluorescence quantitative PCR. Results The levels of TG, TC and LDL-C in HF group were significantly increased (P <0.01) at 12 weeks of feeding with high-fat diet. Compared with HL group, the levels of TG and TC in Pio group were significantly decreased (P <0.01) and the levels of adiponectin in serum were significantly increased (P <0.05). Compared with NC group, the levels of aortic adiponectin receptor 1/2 mRNA expression was significantly decreased (P <0.05); Pio group aortic adiponectin receptor 1/2 mRNA expression was significantly increased (P <0.05 or P <0.01). Conclusion The decrease of adiponectin receptor expression may be involved in vascular injury of hyperlipidemia. Pioglitazone can improve vascular injury and anti - atherosclerosis in hyperlipidemia, which may be related to the increase of adiponectin receptor expression in aorta.