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目的:观察健脾化瘀方体外对人胃癌细胞株SGC-7901凋亡相关基因表达的影响。方法:采用RT-PCR法测定不同浓度健脾化瘀方作用后人胃癌细胞株SGC-7901凋亡相关Bax、Bcl-2、Survivin等基因表达的变化。用Western Blotting测定健脾化瘀方对SGC-7901细胞凋亡相关Bax、Bcl-2蛋白表达的变化。结果:健脾化瘀方大、小剂量组中Bax表达上调,Bcl-2、Survivin表达下调(P<0.05)。结论:健脾化瘀方能通过上调Bax表达,下调Bcl-2、Survivin表达,达到抑制胃癌细胞生长,诱导细胞凋亡的作用。
Objective: To observe the effect of Jianpi Huayu Recipe on the expression of apoptosis-related genes in human gastric cancer cell line SGC-7901 in vitro. Methods: The gene expressions of Bax, Bcl-2 and Survivin in human gastric cancer cell line SGC-7901 were determined by RT-PCR after treated with different concentrations of Jianpi Huayu Fang. Western Blotting determination of Jianpi Huayu Fang SGC-7901 cells apoptosis-related Bax, Bcl-2 protein expression changes. Results: The expression of Bax, Bcl-2 and Survivin in the large and small dose group of Jianpi Huayu were both up-regulated (P <0.05). Conclusion: Jianpi Huayu Recipe can inhibit the growth of gastric cancer cells and induce the apoptosis by up-regulating the expression of Bax and down-regulating the expressions of Bcl-2 and Survivin.