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采用￣（５１）Ｃｒ４小时释放试验，细胞培养及组化分析技术，研究了２６例初诊急性髓细胞白血病患者（ＡＰｓ）和其中的１２例缓解期患者（ＲＰｓ）的ＬＡＫ细胞对自体白血病细胞（Ａｕｔｏ－ＬＢ）的清除作用。ＡＰｓ的外周血或骨髓通过双层Ｆｉｃｏｌｌ－Ｈｙｐａｑｕｅ分离所得富含淋巴细胞层中含２０％～５０％的Ａｕｔｏ－ＬＢ，在ｒＩＬ－２１０００Ｕ／ｍｌ的培养基中培养１～２周Ａｕｔｏ－ＬＢ消失。而￣（５１）Ｃｒ４小时释放试验的结果是ＡＰｓ－ＬＡＫ和ＲＰｓ－ＬＡＫ细胞胞对Ａｕｔｏ－ＬＢ的杀伤活性分别为１２．９％±１２．３％和２４．１％±１２．０％，均较其对Ｒａｊｉ细胞的杀伤活性（３６．０％±３２．０％和６６．２％±１８．１％）显著减低（Ｐ＜０．０１），提示：Ａｕｔｏ－ＬＢ细胞表面能被ＬＡＫ细胞识别的决定簇较Ｒａｊｉ细胞系低。但体外培养过程中Ａｕｔｏ－ＬＢ能被清除。提示：清除机制除了有ＬＡＫ细胞的直接杀伤外，尚有其它机制参与，体外培养过程中Ａｕｔｏ－ＬＢ表面决定簇表达增加。
Using the (51)Cr 4-hour release test, cell culture and histochemical analysis techniques, 26 patients with newly diagnosed acute myeloid leukemia (APs) and 12 patients with remission (RPs) were studied for autologous leukemic cells (LAK cells). Auto-LB) removal. Peripheral blood or bone marrow of APs were separated by double-layer Ficoll-Hypaque to obtain Auto-LB containing 20% to 50% of the lymphocyte-rich layer, and cultured in a medium of rIL-21000U/ml for 1 to 2 weeks. Auto-LB disappeared. . As a result of the (51)Cr 4-hour release test, the killing activities of APs-LAK and RPs-LAK cells on Auto-LB were 12.9%±12.3% and 24.1%±12.0%, respectively. Compared with its killing activity against Raji cells (36.0%±32.0% and 66.2%±18.1%), the killing activity was significantly decreased (P<0.01), suggesting that the surface energy of Auto-LB cells was activated by LAK. Cell-recognition determinants are lower than Raji cell lines. However, Auto-LB can be cleared during in vitro culture. Tip: In addition to the direct killing of LAK cells by the clearance mechanism, there are other mechanisms involved, and the expression of Auto-LB surface determinants increases during in vitro culture.