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为了筛选出辐射效应最佳的非洲菊材料以及最适的辐射剂量,以非洲菊品种“妃子”的愈伤组织、茎尖及组培苗为材料,通过60Co-γ射线进行了0、5、10、20、30 Gy 5种剂量的辐照处理试验。结果表明:在低辐射剂量处理下,非洲菊各处理材料的损伤不明显,反而对茎尖的生长能力表现出一定的促进作用;当辐射剂量逐渐提高时,非洲菊“妃子”的愈伤组织、茎尖和组培苗的最适辐射剂量分别为10~20 Gy、20~30 Gy和20~30 Gy。
In order to screen out the most effective radiation effects Gerbera material and the most suitable radiation dose, the germination of the gerbera “concubine ” callus, shoot tip and tissue culture seedlings as materials, by 60Co-gamma ray was 0, 5, 10, 20, 30 Gy 5 doses of irradiation treatment test. The results showed that under the low radiation dose, the germination of gerbera was not obvious, but the growth ability of shoot tips was enhanced. When the radiation dose was gradually increased, the germination rate of gerbera The optimal dose of irradiation was 10 ~ 20 Gy, 20 ~ 30 Gy and 20 ~ 30 Gy, respectively.