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目的 研究积雪草苷对增生性瘢痕成纤维细胞增殖及对 Smad信号通路的影响。 方法 采用组织块法培养人瘢痕成纤维细胞 ,将成纤维细胞分为用与不用积雪草苷两组 (实验组和对照组 ) ,4 8小时后采用逆转录 -聚合酶链反应 ( reverse transcription- polymerase chain reaction,RT- PCR)观察 Smad2及 Smad7m RNA表达量的改变。应用流式细胞仪分析、免疫细胞化学及蛋白印迹 ( Western blot)技术结合光密度扫描分析 ,观察积雪草苷对瘢痕成纤维细胞周期及磷酸化 Smad2和 Smad7的改变。 结果 实验组可见瘢痕中成纤维细胞从 S期进入 M期细胞减少 ,受到抑制 ;成纤维细胞中 Smad2的含量及 Smad2 m RNA的表达实验组较对照组改变不明显 ,但 Smad7的含量及 Smad7m RNA的表达实验组与对照组分别为 ( 1.33± 1.2 6 ) %、( 5 0 .80± 2 2 .4 0 ) %及 ( 9.15± 3.36 ) %、( 32 .18± 17.84 ) % ,二者两组差异均有统计学意义 ( P<0 .0 5 )。 结论 积雪草苷抑制瘢痕的作用是通过 Smad通路 ,使成纤维细胞增殖受阻而发挥作用的
Objective To study the effect of asiaticoside on the proliferation of hypertrophic scar fibroblasts and its effect on Smad signaling pathway. Methods Fibroblasts were cultured in human scar tissue by tissue block method. The fibroblasts were divided into two groups with and without asiaticoside (experimental group and control group). After 48 hours, reverse transcription-polymerase chain reaction (reverse transcription-) was used. Polymerase chain reaction (RT-PCR) was used to observe the changes of Smad2 and Smad7 mRNA expression. Flow cytometric analysis, immunocytochemistry and western blot were combined with densitometric scanning analysis to observe the changes of cell cycle and phosphorylated Smad2 and Smad7 in asiatic fibroblasts. Results In the experimental group, fibroblasts in the scar decreased from the S phase to the M phase and were inhibited; Smad2 content and Smad2 m RNA expression in the fibroblasts did not change significantly in the experimental group, but Smad7 content and Smad7 mRNA The expression of experimental group and control group were (1.33±1.26)%, (50.80±22.40%) and (9.15±3.36)%, (32.18±17.84)%, respectively. Differences in the groups were statistically significant (P<0.05). Conclusion The effect of asiaticoside in inhibiting scars is through the Smad pathway, which impairs the proliferation of fibroblasts.