目的建立用紫外分光光度法测定尼美舒利栓中尼美舒利的含量。方法用不同的有机溶剂萃取尼美舒利栓中的基质,用紫外分光光度法测定尼美舒利的含量。结果用正己烷作为萃取剂,回收率高(94.4%)。用紫外分光光度法测定尼美舒利栓的含量,标准曲线线性好(r=0.9999)、精密度高(日内RSD=0.47%)、回收率高(98.3%)。结论用紫外分光光度法测定尼美舒利栓剂的含量,方法简便、快速、结果准确,可作为尼美舒利含量测定的方法。 OBJECTIVE To establish a UV spectrophotometric method for the determination of nimesulide in nimesulide suppository. Methods The matrix of nimesulide suppository was extracted with different organic solvents, and the content of nimesulide was determined by UV spectrophotometry. As a result, n-hexane was used as the extractant and the recovery rate was high (94.4%). The content of nimesulide suppository was determined by UV spectrophotometry. The calibration curve has good linearity (r = 0.9999), high precision (RSD = 0.47%) and high recovery (98.3%). Conclusion The determination of nimesulide suppository by UV spectrophotometry is simple, rapid and accurate. It can be used as a method for the determination of nimesulide content.