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为研究冷冻对人精子生育力及染色体的影响。本实验选用健康、已生育、２５～３５岁男性供精者的精液，以７．５％的甘油作冷冻保护剂，通过冷冻前后精液常规分析、穿卵试验、染色体核型分析，发现在冷冻－复苏后，精子活动率、穿卵率均有一定程度下降，精子染色体数目和结构异常的发生频率冻贮前后无显著差异。但在活力好、具有生育能力的精子中，携Ｘ－染色体的精子与携Ｙ－染色体的精子所占比例发生了显著改变，由冻前的Ｘ：Ｙ＝５０： ５０变成冻后的 ６０．８５： ３９．１５。
To study the effects of freezing on human sperm fertility and chromosomes. In this experiment, healthy, fertile, 25 ~ 35-year-old male sperm for semen, with 7.5% glycerol as cryoprotectants, before and after freezing by semen routine analysis, egg penetration test, chromosome karyotype analysis and found in the frozen - After resuscitation, sperm motility rate and the rate of weaning rate all decreased to a certain extent. There was no significant difference in the frequency of sperm chromosome number and structure abnormality before and after freezing. However, among the viable and fertile spermatozoa, the proportion of X-chromosome bearing sperm and Y-chromosome bearing sperm changed significantly from X: Y = 50:50 before freezing to 60 .85: 39.15.