论文部分内容阅读
目的 :探讨视黄醛酸 β受体 (RAR β)及半乳糖凝集素 8(galectin 8)在中耳继发性胆脂瘤上皮的表达 ,阐明这两种因子在胆脂瘤发病机制中的作用。方法 :分别应用免疫组织化学和WesternBlot技术检测RAR β和 galectin 8在 4 2例胆脂瘤和 18例胆脂瘤外耳道皮肤中的表达情况。 结果 :RAR β在 4 2例胆脂瘤上皮各层细胞均存在较强表达 ,定位于胞膜和胞质 ,外耳道皮肤表达较弱 ;两种组织RAR β阳性表达的平均 A值分别为0 .2 734± 0 .0 0 2 2和 0 .0 12 4± 0 .0 0 14 ,差异有统计学意义 (t =34.33,P <0 .0 1)。galectin 8在胆脂瘤上皮组织的浅表层和基底层的表达无明显区别 ,均为胞质和胞膜出现棕黄色颗粒 ,结缔组织中同样如此 ,平均A值为0 .2 82 6± 0 .0 0 16 ;而对照组只有弱阳性表达 ,平均A值为 0 .0 394± 0 .0 0 0 14 ,差异有统计学意义 (t =4 0 .89,P<0 .0 1)。同时我们还比较了RAR β和 galectin 8表达的相关性 ,发现两者的表达高度相关 (P <0 .0 1)。结论 :中耳胆脂瘤可能是一群幼稚未分化的角质细胞无限制地增殖的结果 ;galectin 8的表达水平与RAR β存在潜在相关性
Objective: To investigate the expression of retinal β receptor (RAR β) and galectin 8 in the secondary middle ear cholesteatoma epithelium and elucidate the roles of these two factors in the pathogenesis of cholesteatoma effect. Methods: The expression of RAR β and galectin 8 in 42 cases of cholesteatoma and 18 cases of cholesteatoma were evaluated by immunohistochemistry and Western Blot respectively. RESULTS: RARβ was strongly expressed in all the layers of 42 cholesteatoma epithelial cells and located in the membrane and cytoplasm. The expression of RARβ in the skin of external auditory canal was weak. The average A values of RAR β in both tissues were 0 and 0 respectively. 2 734 ± 0 .0 022 and 0 024 ± 0 0 0 14, the difference was statistically significant (t = 34.33, P <0.01). The expression of galectin 8 in the superficial and basal layers of cholesteatoma epithelium was not significantly different, both of the cytoplasm and the cell membrane appeared brown granules, the same was true in the connective tissue, with an average A value of 0.82 6 ± 0. While the control group only weakly positive expression, the average A value of 0.0394 ± 0.0604, the difference was statistically significant (t = 4.089, P <0.01). We also compared the expression of RAR β and galectin 8, and found that the expression of RAR β and galectin 8 were highly correlated (P <0.01). CONCLUSIONS: Cholesteatoma of the middle ear may be the result of unlimited proliferation of naive undifferentiated keratinocytes; the expression level of galectin 8 is potentially associated with RAR β