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目的探讨胆管细胞性肝癌(CCC)与乙型肝炎病毒(HBV)感染间的关系。方法应用PCR-EB法对胆管细胞性肝癌、胆管结石的肝组织、石蜡包埋标本进行HBV-DNA检测,并回顾性地与ELISA法检测的血清HBV五项标志物进行对比。结果 HBV-DNA阳性率在胆管细胞性肝癌组明显高于肝胆管结石组(P<0.01);胆管细胞性肝癌的癌旁胆管上皮细胞异型增生HBV-DNA阳性组明显高于HBV-DNA阴性组(P<0.01)。结论胆管细胞性肝癌与HBV感染有关,其发病过程为HBV感染→慢性肝炎→胆管上皮细胞异型增生→肝癌。
Objective To investigate the relationship between cholangiocarcinoma (CCC) and hepatitis B virus (HBV) infection. Methods PCR-EB method was used to detect HBV-DNA in liver tissue and paraffin-embedded specimens of cholangiocarcinoma, bile duct stones, and retrospectively compared with five serum markers of HBV detected by ELISA. Results The positive rate of HBV-DNA was significantly higher in the cholangiocarcinoma group than in the hepatolithiasis group (P<0.01). The HBV-DNA positive group was significantly higher in the para-tumor bile duct epithelial cells than in the HBV-DNA negative group. (P<0.01). Conclusion The cholangiocarcinoma is associated with HBV infection. The pathogenesis is HBV infection → chronic hepatitis → dysplasia of bile duct epithelial cells → liver cancer.