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以黄连素及其化学衍生物为分析对象，通过不同操作条件下分离效能、溶质迁移时间、电压电流关系以及电泳运行功率的关系，来考察高效毛细管电泳过程中管内焦耳热现象对样品分离的影响，明确了在综合优化电泳分离条件中限制焦耳热的必要性。
Taking the berberine and its chemical derivatives as the analysis objects, the influence of Joule heating in the tube on the sample separation during high performance capillary electrophoresis was investigated by the separation efficiency, solute transport time, voltage-current relationship and electrophoresis operating power under different operating conditions , Clarified the necessity of limiting Joule heating in a comprehensive and optimized electrophoretic separation condition.