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目的:分析抗菌肽人β防御素3(humanβdefensin 3,hBD3)对铜绿假单胞菌PAO-1株的抑制作用。方法:合成抗菌肽hBD3,分别通过最低抑菌浓度(minimal inhibitory concentration,MIC)检测、直接杀菌试验、重要功能基因检测分析其对PAO-1的直接抑制作用;并将其与阿奇霉素、四环素、利福平、氯霉素、链霉素、环丙沙星联合施用,观察对抗生素MIC的影响。结果:HBD3对PAO-1的MIC为32μg/mL;在浓度达到8μg/mL时即有明显杀菌作用。HBD3上调PAO-1株的ahpF基因表达,下调aprA和rhlR基因表达。在联用5μg/mL的hBD3后,四环素、利福平、氯霉素、链霉素、环丙沙星的MIC值均有降低。结论:抗菌肽hBD3对铜绿假单胞菌PAO-1株有显著的抑制作用。
Objective: To investigate the inhibitory effect of human βdefensin 3 (hBD3) on PAO-1 strain of Pseudomonas aeruginosa. Methods: Antibacterial peptide hBD3 was synthesized and tested for its direct inhibitory activity against azithromycin, tetracycline and tetracycline by the methods of minimal inhibitory concentration (MIC), direct bactericidal test and important functional gene test, respectively Fuping, chloramphenicol, streptomycin, ciprofloxacin combined administration, observed on the impact of antibiotic MIC. Results: The MIC of HBD3 to PAO-1 was 32μg / mL. At the concentration of 8μg / mL, the bactericidal effect was obvious. HBD3 up-regulated the ahpF gene expression of PAO-1 strain and down-regulated aprA and rhlR gene expression. MICs of tetracycline, rifampicin, chloramphenicol, streptomycin and ciprofloxacin were all decreased after 5μg / mL hBD3 was combined. Conclusion: Antimicrobial peptide hBD3 has a significant inhibitory effect on Pseudomonas aeruginosa PAO-1 strain.