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人类免疫缺陷病毒(Humanimmunodeficiencyvirus,HIV)附属蛋白Nef、Vpu、Vpr和Vif在病毒复制中起着关键作用,并能被细胞毒性T细胞(CytotoxicTLymphocyte,CTL)识别。然而,对我国HIV感染者体内附属蛋白特异性的CTL应答研究比较少。本研究应用覆盖HIV-1B、C亚型附属蛋白(Nef、Vpu、Vpr和Vif)的142个肽段作为抗原,通过酶联免疫斑点实验(Enzyme-LinkedImmunospot,ELISPOT)检测61例中国HIV/AIDS患者和10例HIV-1血清阴性对照的HIV-1附属蛋白特异性CTL应答。无论对HIV-1B亚型还是HIV-1C亚型附属蛋白都能产生特异性CTL应答,特别是Nef区蛋白的反应频率和累积应答强度都较高(P<0.001),B、C亚型间的应答频率和累积应答强度都无显著差别(P>0.05),其免疫优势区也大致相同。附属蛋白特异性的累积CTL应答强度将近达到总应答的21%。这些结果表明尽管HIV-1附属蛋白的体积小,但它们在诱导特异性的CTL应答中发挥了重要作用,对评价HIV-1免疫应答的幅度和特异性以及研发针对中国人群的HIV疫苗有重要的意义。
Human immunodeficiency virus (HIV) accessory proteins Nef, Vpu, Vpr and Vif play a key role in viral replication and can be recognized by cytotoxic T lymphocytes (CTLs). However, there are few studies on the CTL response specific to the ancillary proteins in vivo in HIV-infected people in China. In this study, 142 peptides of HIV-1B and C subtype C (Nef, Vpu, Vpr and Vif) were used as antigen to detect 61 cases of HIV / AIDS in China by Enzyme Linked Linked Immunospot (ELISPOT) HIV-1 adjuvant-specific CTL responses of patients and 10 HIV-1 seronegative controls. The specific CTL responses of both the HIV-1 subtype and the subtype of HIV-1 C subtype were found. In particular, the Nef region protein response frequency and cumulative response intensity were higher (P <0.001) There was no significant difference in response frequency and cumulative response intensity (P> 0.05). The immunodominant areas were also roughly the same. The accessory protein-specific cumulative CTL response intensity nearly reached 21% of the total response. These results indicate that despite the small size of HIV-1 adjuvant proteins, they play an important role in inducing specific CTL responses and are important for assessing the magnitude and specificity of the HIV-1 immune response and developing HIV vaccines against the Chinese population Meaning.