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目的了解HIV抗体筛查阳性结果与确证结果之间的关系,为HIV感染诊断提供科学依据。方法采用酶联免疫法和胶体硒法对样本进行HIV抗体筛查,筛查阳性样本进行确证试验(WB),对筛查结果和确证结果进行比较分析。结果 708份HIV抗体筛查阳性样本,确证试验HIV-1抗体阳性629份,占88.84%;不确定57份,占8.05%;阴性22份,占3.11%;629份确证阳性样本中,626份ELISA法S/CO值>6,占99.52%,S/CO值<1确证结果无阳性;确证阳性样本抗env基因编码蛋白阳性率最高,gp160和gp120的阳性率均为100%;不确定样本中,gp160和p24出现的几率最高,分别为77.19%和68.42%。结论 HIV抗体筛查试验存在一定的假阳性,S/CO值>6的样本确证阳性的几率较高;对同时出现gp160和p24的不确定样本应予以重视。
Objective To understand the relationship between the positive results of HIV antibody screening and the confirmatory results and provide a scientific basis for the diagnosis of HIV infection. Methods Enzyme-linked immunosorbent assay (ELISA) and colloidal selenium method were used to screen HIV antibodies and positive samples were screened for confirmatory test (WB). The results of screening and confirmatory tests were compared and analyzed. Results A total of 708 HIV antibody positive samples were tested positive for HIV-1 antibody, accounting for 88.84% of the total HIV-1 antibody positive samples, 57 of them were undefined (8.05%), 22 were negative (3.11%), 626 ELISA method S / CO value> 6, accounting for 99.52%, S / CO value <1 to confirm the results were positive; positive samples confirmed the highest positive rate of anti-env gene encoding protein, gp160 and gp120 positive rate was 100%; uncertain samples Among them, gp160 and p24 showed the highest probability of 77.19% and 68.42% respectively. Conclusion HIV antibody screening test has some false positives. The samples with S / CO value> 6 are more likely to be confirmed positive. And the uncertain samples with both gp160 and p24 should be taken seriously.