目的采用南昌大学第一附属医院血液科自行研制的无血清培养基血清代用品替代血清在体外诱导培养急性白血病细胞生成 DCs,以寻找一种适合于临床应用的 DCs 体外诱导培养方法。方法 13例急性髓系白血病(AML)均为初发患者,其中 M_13例,M_2 4例,M_3 3例,M_4 2例,M_5 2例。白血病细胞在含1000 U/mL,GM-CSF、1000 U/mLIL-4、500 U/mL TNF-α、25 U/mLbFGF 的无血清培养基 Objective To study the in vitro culture method of DCs which is suitable for clinical application by using serum-free serum-substitute serum developed by the Department of Hematology, the First Affiliated Hospital of Nanchang University as a substitute for serum to induce the formation of DCs in acute leukemia cells in vitro. Methods Thirteen patients with acute myeloid leukemia (AML) were all newly diagnosed patients, including M13 cases, M_2 cases, M_3 cases, M_42 cases and M_5 cases. Leukemia cells were cultured in serum-free medium containing 1000 U / mL, GM-CSF, 1000 U / mL IL-4, 500 U / mL TNF-α, 25 U / mL bFGF