【摘 要】
：
Earthworms(Eiseniafetida)were used to study the impact of low-dose cadmium in treated artificial soil(0,0.6,3,6,15,30 mg/kg)and contaminated natural soil(1.46 m
【机 构】
：
Department of Ecology Science and Engineering,China Agricultural University,Beijing 100193,China;Sta
【基金项目】
：
the Key Beijing Discipline of Ecology;国家自然科学基金;the National Key Technology R&D Program in the 11th Five-Year Plan of China
论文部分内容阅读
Earthworms(Eiseniafetida)were used to study the impact of low-dose cadmium in treated artificial soil(0,0.6,3,6,15,30 mg/kg)and contaminated natural soil(1.46 mg/kg).The changes of earthworms’ physiological related gene expressions of metallothionein (MT),annetocin,calreticulin and antimicrobial peptides were detected using real-time PCR after a 70-day incubation period.The results showed that low doses of cadmium could up regulate earthworms’ MT and down regulate armetocin gene expression and show a significant positive and negative correlation respectively.The expression of two other genes,calreticulin and anti-microbial peptides,was induced at low doses of cadmium(highest gene expression at 0.6 mg/kg for calreticulin and 6 mg/kg for anti-microbial peptides)and inhibited at high doses.No significant correlation was found for these two genes.This study shows that MT and annetocin genes expression found in earthworms in contaminated soil have the potential to be developed as biomarkers of soil cadmium pollution.
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