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目的探讨1,2-二甲基-3-羟基-4-吡啶酮(1,2-dimethyl-3-hydroxy-4-pyridone,deferiprone,DFP)对铝染毒小鼠血清蛋白和肝脏酶活力的影响。方法将50只健康清洁级昆明小鼠按体重随机分为5组,分别为阴性对照(生理盐水)组、铝(200 mg/kg)单独染毒组和低(17.58 mg/kg)、中(35.15 mg/kg)、高(70.30 mg/kg)剂量DFP+铝(200 mg/kg)染毒组,每组10只,雌雄各半。第1～4周,灌胃染毒AlCl3溶液,染毒容量为10 ml/kg,每天1次,每周连续5 d;第5周,灌胃染毒DFP溶液,染毒容量20 ml/kg,每天1次,连续5 d。测定血清中总蛋白(TP)、白蛋白(Alb)含量和γ-谷氨酰转移酶(γ-GT)活力以及肝脏中铝含量和ATP酶、黄嘌呤氧化酶(xanthine oxidase,XO)的活力。结果与阴性对照组比较,铝单独染毒组肝脏铝含量较高(P<0.01),血清TP含量较低(P<0.01),给予DFP后各组均恢复至阴性对照组水平(P>0.05);铝单独染毒组血清Alb含量比阴性对照组降低(P<0.05),给予中、高剂量DFP后恢复至阴性对照组水平(P>0.05);与阴性对照组比较,铝单独染毒组和低、中剂量DFP+铝染毒组肝脏Ca2+-Mg2+-ATP酶活力均较低(P<0.01),XO活力较高(P<0.01),仅高剂量DFP+铝染毒组肝脏Ca2+-Mg2+-ATP酶活力与阴性对照组无差异(P>0.05),低、中、高剂量DFP+铝染毒组XO活力低于铝单独染毒组(P<0.05,P<0.01),且高剂量DFP+铝染毒组与阴性对照组无差异(P>0.05)。结论服用DFP对铝染毒小鼠血清蛋白和肝脏酶活力的改变具有一定的恢复作用。
Objective To investigate the effects of 1,2-dimethyl-3-hydroxy-4-pyridone (DFP) on the activities of serum protein and liver enzymes in aluminum-exposed mice influences. Methods Fifty healthy and clean Kunming mice were randomly divided into five groups according to body weight: negative control (saline), aluminum (200 mg / kg) alone and low (17.58 mg / kg) 35.15 mg / kg) and DFP + aluminum (200 mg / kg) at doses of 70.30 mg / kg. The first to fourth week, intragastric administration of AlCl3 solution, exposure capacity of 10 ml / kg, once a day for 5 consecutive weeks; the first 5 weeks, intragastric administration of DFP solution, the exposure capacity of 20 ml / kg , 1 day, for 5 days. Serum total protein (TP), albumin (Alb) and γ-glutamyl transferase (γ-GT) activity and aluminum content in the liver and the activity of ATPase and xanthine oxidase (XO) . Results Compared with the negative control group, the aluminum content in liver of aluminum-exposed group was significantly higher (P <0.01) and lower in serum TP (P <0.01) ). The level of Alb in Al-alone group was lower than that in the negative control group (P <0.05), and returned to the level of negative control group after middle and high dose of DFP (P> 0.05). Compared with the negative control group, The activities of Ca2 + -Mg2 + -ATPase in the liver and the XO activity in the DFP + aluminum-exposed group were lower than those in the DFP + aluminum-treated group (P <0.01) (P <0.05). The activities of XO in low, middle and high dose DFP + aluminum exposure groups were lower than those in aluminum alone exposure group (P <0.05, P <0.01), and high DFP + There was no difference between aluminum exposure group and negative control group (P> 0.05). Conclusion DFP has a certain effect on the changes of serum protein and liver enzyme activity in aluminum-exposed mice.