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在室内饲养的水椰八角铁甲Octodonta nipae(Maulik)种群中,发现有大量甲虫被病原菌感染致死。对死虫体表的病原真菌进行分离鉴定,并依据ITS序列分析鉴定,确定该病原真菌为金龟子绿僵菌小孢变种(Metarhizium anisopliae var.anisopliae)。经室内致病力测定,接种浓度分别为1.0×105～1.0×1010cfu·mL-1孢子悬浮液,在接菌后10d,成虫致病力方程为y=-4.992+5.623x,LC50对数剂量为7.721;幼虫致病力方程为y=-4.335+5.368x,LC50对数剂量为6.420。结果表明金龟子绿僵菌小孢变种对水椰八角铁甲幼虫和成虫具有高的致病力,且浓度越高感染率越高,相同浓度条件下幼虫的感染率较成虫高。金龟子绿僵菌小孢变种在水椰八角铁甲生物防治中具有较强的应用潜力。
Inside the Octodonta nipae (Maulik) population of indoor octopus, a large number of beetles were found dead by pathogenic bacteria. The pathogenic fungi on the surface of dead insects were isolated and identified, and based on ITS sequence analysis identified to determine the pathogenic fungi Metarhizium anisopliae var. Anisopliae. The indoor pathogenicity was determined by inoculation concentration of 1.0 × 105 ~ 1.0 × 1010cfu · mL-1 spore suspension, 10d after inoculation, the adult pathogenicity equation was y = -4.992 + 5.623x, LC50 logarithmic dose Was 7.721. The larval pathogenicity equation was y = -4.335 + 5.368x, and the LC50 logarithmic dose was 6.420. The results showed that Metarhizium anisopliae Metarhizium anisopliae had a high virulence to larvae and adult larvae of Acanthurium nivalis, and the higher the infection rate was, the higher the infection rate of larvae was. Metarhizium Metarhizium spores Variegated Crab horn biological control has a strong potential.