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目的:建立HPLC法测定阿巴卡韦双夫定片中阿巴卡韦、拉米夫定、齐多夫定的含量。方法:采用Hypersil C18(200mm×4.6mm,5μm)色谱柱,以甲醇-庚烷磺酸钠溶液(取庚烷磺酸钠2.02 g,加三乙胺5 ml,加水至700 ml,用磷酸调p H至3.9)(30∶70)为流动相,流速为1.0 ml·min-1,检测波长270 nm。结果:拉米夫定、齐多夫定、阿巴卡韦分别在0.08~1.60μg(r=0.999 4),0.24~4.80μg(r=0.999 9),0.24~4.80μg(r=0.999 9)范围内线性关系良好,平均回收率分别为99.6%(RSD=0.9%),99.7%(RSD=0.9%),99.9%(RSD=1.0%)。结论:本方法简便、可靠、准确,可用于同时测定三组分含量。
OBJECTIVE: To establish an HPLC method for the determination of abacavir, lamivudine and zidovudine in abacavir-Shuangfuding tablets. METHODS: Hypersil C18 (200 mm × 4.6 mm, 5 μm) column was used with methanol-heptane sulfonate solution (2.02 g of sodium heptane sulfonate, 5 ml of triethylamine and 700 ml of water, adjusted with phosphoric acid pH to 3.9) (30:70) as the mobile phase at a flow rate of 1.0 ml · min-1 at a detection wavelength of 270 nm. Results: The lamivudine, zidovudine, and abacavir ranged from 0.08 to 1.60 μg (r = 0.999 4), 0.24 to 4.80 μg (r = 0.999 9), 0.24 to 4.80 μg (r = 0.999 9) The linearity was good with an average recovery of 99.6% (RSD = 0.9%), 99.7% (RSD = 0.9%) and 99.9% (RSD = 1.0%), respectively. Conclusion: The method is simple, reliable and accurate and can be used for simultaneous determination of three components.