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本研究在不同p H和温度条件下以离体方式对野生棘胸蛙(Quasipaa spinosa)不同消化器官的蛋白酶活力进行了分析。结果表明,食道、胃、胰的蛋白酶活力受到酶体系p H的显著影响(P<0.05),且随p H升高呈现典型的单峰型活力曲线。食道和胃的蛋白酶活力在p H为1.5时达到峰值,胰的酶活力在p H为9.6时达到峰值,而肠道酶活力在p H为7.4时达到峰值。各消化器官蛋白酶活力具有明显的温度依赖性(P<0.05),酶活力随温度升高也均呈典型的单峰型活力曲线,不同消化器官的最大酶活力温度分别为,食道50℃、胃50℃、胰45℃、前肠45℃、后肠45℃、直肠45℃。在最大酶活力的p H和30℃条件下,各消化器官蛋白酶活力由高到低依次为胰、食道、胃、直肠、前肠、后肠。由此可见,蛋白酶在棘胸蛙消化系统的分布具有明确的规律性,且不同来源的蛋白酶需要在特定p H和温度下才能表现出最大的反应活性。
In this study, the protease activities of different digestive organs of Quasipa spinosa were analyzed ex vivo under different pH and temperature conditions. The results showed that the protease activity in the esophagus, stomach and pancreas was significantly affected by the p H of the enzyme system (P <0.05), and showed a typical single peak activity curve with increasing p H. Protease activity in the esophagus and stomach peaked at a p H of 1.5, with pancreatic enzyme activity reaching a peak at p H of 9.6 while intestinal enzyme activity peaked at a p H of 7.4. The activity of protease in each digestive organ was significantly temperature-dependent (P <0.05). The activity of the digestive organ also showed a typical unimodal activity curve with increasing temperature. The maximal enzymatic activities of different digestive organs were 50 ℃, 50 ° C, pancreas 45 ° C, foregut 45 ° C, hindgut 45 ° C, rectum 45 ° C. Under the conditions of the maximum enzyme activity of p H and 30 ℃, the activity of digestive organ protease descending order of pancreas, esophagus, stomach, rectum, foregut and hindgut. Thus, the distribution of protease in the digestive system of Spinach frog has a clear regularity, and proteases of different origins need to exhibit maximum reactivity at specific p H and temperature.