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[目的]通过研究了解叶施和根灌ALA对低温胁迫下香蕉幼苗的影响。[方法]用4种不同浓度ALA分别叶施和根灌巴西蕉幼苗后置于人工气候箱中7℃下模拟低温胁迫,研究ALA不同处理方式对巴西蕉幼苗冷害的影响。[结果]结果表明,无论叶施或根灌,ALA均可提高低温胁迫下香蕉幼苗叶片超氧化物歧化酶(SOD)、过氧化物酶(POD)活性,降低超氧阴离子产生速率、MDA含量及相对电导率,同时还可增加脯氨酸、可溶性蛋白含量及相对含水量(RWC),维持叶绿素的稳定性。以叶施0.50~5.00mg/LALA效果最好,与同浓度根灌相比,叶施处理的叶绿素含量均显著增加,相对电导率、丙二醛含量均显著降低,5mg/LALA叶施比根灌SOD和POD活性提高了64.5%、12.3%,RWC提高了12.1%,差异均达显著水平。[结论]由此表明,ALA叶面喷施可更好地减缓低温胁迫对香蕉造成的冷害。
[Objective] The research aimed to understand the effects of leaf and root irrigation ALA on banana seedlings under chilling stress. [Method] Leaf explants of Arabidopsis thaliana (Brassica campestrisch) were pruned and primed with four different concentrations of ALA respectively and then placed in an artificial climate chamber at 7 ℃ for simulated low temperature stress to study the effects of different treatments of ALA on the chilling injury of Brazilian banana seedlings. [Result] The results showed that ALA could enhance the activities of superoxide dismutase (SOD) and peroxidase (POD), decrease the production rate of superoxide anion and the content of MDA in leaves of banana seedlings under low temperature stress And relative conductivity, but also increased proline, soluble protein content and relative water content (RWC), to maintain the stability of chlorophyll. Leaf application of 0.50 ~ 5.00mg / LALA was the best, compared with the same concentration of root irrigation, leaf chlorophyll content were significantly increased, the relative conductivity, malondialdehyde content were significantly reduced, 5mg / LALA leaves than the root The activities of SOD and POD were increased by 64.5%, 12.3% and RWC by 12.1%, respectively, with significant differences. [Conclusion] The results showed that foliar application of ALA could alleviate the chilling injury of banana under low temperature stress.