论文部分内容阅读
本文对β-内酰胺酶的多种国内外检测方法进行了介绍,并着重比较了杯碟法和酶热仪法的特点,详细分析了两种方法在检测原理、仪器设备、实验操作、实验周期、结果判定、方法学参数等方面的异同点。杯碟法稳定可靠、灵敏度高,是目前中国乳及乳制品中β-内酰胺酶检测的推荐方法;酶热仪法简便快捷、可实现半定量检测,更适合于大规模样品筛查和现场检测。两种方法的测定结果具有可比性,可根据检测要求和实验室情况配合使用。
In this paper, a variety of detection methods of β-lactamase at home and abroad are introduced, and the characteristics of cup-dish method and enzyme-thermo-meter method are emphatically compared. The principle, equipment, experimental operation, experiment Cycle, the results of judgment, methodological parameters and other aspects of the similarities and differences. Cup dish method is stable and reliable, high sensitivity, is currently China’s milk and dairy products β-lactamase detection of recommended methods; Enzyme method is simple and fast, semi-quantitative detection can be achieved, more suitable for large-scale sample screening and on-site Detection. The determination results of the two methods are comparable and can be used according to the testing requirements and laboratory conditions.