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应用补体介导的微量淋巴细胞毒实验可检出ＨＬＡＣｗ１～１０，但有２０％～５０％的空白（ｂｌａｎｋ）不能检出。利用ＰＣＲＳＳＰ对７０例上海汉族样本进行ＨＬＡＣｗ等位基因分型，首次在中国汉族人群中检出Ｃｗ１２０１／１２０２、１２０３、１３０１、１４、１６０１、１６０２、１７０１等非血清学鉴定的抗原，并对血清学鉴定抗原的基因进行分型，依次为Ｃｗ０１、０２、０３０２／０３０４、０３０３、０４、０５０１、０６０２、０７０１／０７０２／０７０３及０８。ＰＣＲＳＰ分型使上海汉族人群ＨＬＡＣｗ位点空白基因频率从０．３０７下降到０．０２８。１２例样本检出Ｃｗ０８，与原来认为中国人群中Ｃｗ８罕见的观点不符。结果证明，ＰＣＲＳＳＰ用于ＨＬＡＣｗ基因分型是快速、准确的。
Application of complement-mediated trace lymphocytotoxicity test can be detected HLA CW1 ~ 10, but 20% to 50% of the blank (blank) can not be detected. PCR-SSP 70 cases of Shanghai Han sample HLA CW allele, the first Chinese Han population detected Cw * 1201 1202,1203,1301,14,1601,1602,1701 and other non-serological identification Of the antigens, and then typed the genes of the serologically identified antigens, followed by Cw * 01,02,0302 / 0304,0303,04,0501,0602,0701 / 0702 / 0703,08. PCRSP typing reduced the frequency of HLA-Cw locus blank genes in Shanghai Han population from 0.307 to 0.028.12 samples were detected Cw 08, inconsistent with the original view that the Chinese population Cw8 rare. The results show that, PCR SSP for HLA Cw genotyping is fast and accurate.