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目的 比较口腔鳞癌肿瘤浸润淋巴细胞(tumor infiltrating lymphocyte,TIL)和肿瘤引流淋巴结细胞(tumordraining lymph node lymphocyte,TDLNL)体外杀伤活性。方法 从5例口腔癌原发灶及其转移灶中分离TIL和TDLNL，用3H-TdR4h释放法对TDLNL和TIL对自体肿瘤的杀伤活性进行比较研究。结果 3周时TIL对自体肿瘤的杀伤活性较TDLNL强(P<0.02)，分别为35%和23%；4周时TDLNL对自体肿瘤的杀伤活性大于TIL(P<0.02)，分别为53%和40%；5周时TDLNL对自体肿瘤的杀伤活性仍显著强于TIL(P<0.02)，分别为62%和44%。结论 在口腔鳞癌过继免疫治疗中，TDLNL可能较TIL具有更大的优势。“,”Objective To compare the cytotoxicity of TIL and TD LNL frompatients with oral squamous cell carcinoma. Methods TILs were isolated from fresh tumor tissues in 5 OSCC Opati e nts with metastases and TDLNL were obtained from metastatic lymph nodes of them. Then TIL and TDLNL were cultured in the presence of 1000u/ml rIL-2. The cytoto xicity against autologous tumor cells of TIL and TDLNL were detected comparably using 3H-TdR 4 h-release mehtod. Results The cytotoxicity against autologous tumor cells of TIL was stronger than that of TDLNL (P<0.02) cultured for 3 weeks, were 35 percent and 23 percent respectively. When cultured for 4 weeks, the latter became stronger than the former (P<0.02), were 53 percent and 40 percent respectively. Cultured for 5 weeks, the latter still exhibited significantly higher than the f ormer (P<0.02). Conclusions TDLNL seems possessed more superiority than TIL as AIT for patients with OSCC.