早期未分化造血干细胞是由不均一的、有不同程度再生能力、系列定向和分化状态的细胞组成。本文应用选择性的密度梯度荧光激活细胞分离仪(FACS)分离极早期造血细胞亚群,可获得浓集的、已清除掉定向干细胞的骨髓原始细胞。这些细胞有母细胞光散射特征,与DNA结合染料H(?)eshst33342(H(?))有低水平结合,与线粒体染料罗丹明(Rh-123)有不同量的结合。这些染料用于顺序单一 Early undifferentiated hematopoietic stem cells are heterogeneous, with varying degrees of regeneration, a series of directional and differentiated state cells. In this paper, a selective density-gradient fluorescence activated cell sorter (FACS) was used to isolate very early hematopoietic subpopulations and to obtain enriched bone marrow blast cells with cleared stem cells. These cells have the characteristics of light scattering of the mother cell, low level binding with the DNA binding dye H (?) Eshst33342 (H (?)) And different amount of binding with the mitochondrial dye rhodamine (Rh-123). These dyes are used in a single order