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目的研究2周的尾吊复合隔离环境所导致的大鼠抑郁样行为。方法 24只雄性SD大鼠随机平均分为3组:对照(Con)、尾吊(tail suspension,TS)、尾吊复合隔离(tail suspension combined with isolation,TS+I)。造模前后检测摄食量和体重变化,造模结束后依次进行空场、新奇事物探索、强迫游泳实验。结果 TS和TS+I组大鼠体重和摄食量低于对照组,且TS+I组最低。TS和TS+I组大鼠的主动性活动显著少于对照组,且TS+I组大鼠最少。TS和TS+I组大鼠探索潜伏期变长,且探究行为减少,其中TS+I组大鼠表现最差。TS和TS+I组大鼠的攀爬次数少于对照组,TS+I组大鼠不动时间最长。结论 2周的尾吊复合隔离环境导致的抑郁样行为比单一尾吊更为严重,此模型可用于航天抑郁发病机制研究及对抗药物开发。
Objective To study the depression-like behavior of rats induced by 2-week tail-hang compound isolation environment. Methods Twenty-four male Sprague Dawley rats were randomly divided into three groups: control (Con), tail suspension (TS) and tail suspension combined with isolation (TS + I). Before and after the model of food intake and body weight changes were measured, followed by the empty field after modeling, novelty to explore, forced swimming experiment. Results The body weight and food intake of rats in TS and TS + I group were lower than those in control group, and TS + I group was the lowest. The activity of TS and TS + I groups was significantly less than that of the control group, and TS + I group was the least. TS and TS + I group rats to explore the latent period longer, and to explore the behavior decreased, TS + I group rats showed the worst. TS and TS + I group rats climbing less than the control group, TS + I group rats immobility time is the longest. Conclusions The two-week tail-hang composite isolation environment led to more depression-like behavior than the single tail hang. This model can be used to study the pathogenesis of space depression and to combat drug development.