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目的研究长期酒精摄入对大鼠脂肪组织肿瘤坏死因子α(TNF α)及瘦素(Leptin)mRNA表达水平的影响,探讨酒精导致胰岛素抵抗的机制。方法根据给予酒精的剂量0,0.8,1.6,2.4g·kg~(-1)·bw~(-1)。将Wistar大鼠40只分为对照组(Con)和低剂量酒精(LA)、中剂量酒精(MA)、高剂量酒精(HA)组,测血糖、胰岛素、血TNF-α和Leptin浓度,计算胰岛素抵抗指数,RT-PCR方法测定TNF-α,Leptin mRNA表达水平。结果与Con组相比,各剂量组血糖呈酒精剂量依赖性升高(r=0.499,P<0.01),各剂量组HOMA-IR增加且差异均具有统计学意义(P<0.05);各剂量组血清中TNF-α浓度及脂肪组织中TNF α mRNA表达水平增加,HA组差异有统计学意义(P<0.05);各剂量组血清Leptin浓度及脂肪组织Leptin mRNA表达水平均无改变。结论脂肪组织TNF-α mRNA表达水平升高可能是酒精导致胰岛素抵抗分子机制之一。
Objective To investigate the effect of long-term alcohol intake on TNFα and leptin mRNA expression in adipose tissue of rats and to explore the mechanism of alcohol-induced insulin resistance. Methods According to the dose of alcohol given 0,0.8,1.6,2.4 g · kg -1 · bw -1. 40 Wistar rats were divided into control group (Con), low dose alcohol (LA), middle dose alcohol (MA), high dose alcohol (HA) group, blood glucose, insulin, blood TNF- Insulin resistance index, RT-PCR method for determination of TNF-α, Leptin mRNA expression levels. Results Compared with Con group, blood glucose in each dose group increased in a dose-dependent manner (r = 0.499, P <0.01), HOMA-IR increased and the difference was statistically significant (P <0.05) The concentration of TNF-α in serum and the expression of TNFα mRNA in adipose tissue were increased in HA group (P <0.05). There was no change in serum Leptin concentration and Leptin mRNA expression in adipose tissue in each dose group. Conclusion The increased expression of TNF-α mRNA in adipose tissue may be one of the molecular mechanisms of alcohol-induced insulin resistance.