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目的:研究安络化纤丸对实验性血吸虫病肝纤维的影响,探讨其相关作用机制。方法:将36只昆明小鼠随机分为正常对照组、感染对照组及安络化纤丸组,采用日本血吸虫尾蚴40条/只攻击感染小鼠建立血吸虫性肝纤维化模型,用安络化纤丸干预连续8周,观察肝组织纤维化程度及虫卵结节变化;SABC免疫组织化学检测αSMA及TIMP-1的表达。结果:安络化纤丸组小鼠肝组织虫卵肉芽肿个数较感染对照组少,虫卵肉芽肿平均直径较感染对照组小,小鼠肝组织TIMP-1及αSMA的水平较感染对照组显著下降,(P<0.05)。结论:安络化纤丸可减小虫卵肉芽肿的直径,抑制肝纤维化的形成,其机制可能是抑制肝星状细胞活化,减少TIMP-1的表达而达到抗纤维化的目的。
Objective: To study the effect of Anluohuaxian pill on hepatic fibrosis of experimental schistosomiasis and to explore its related mechanism. Methods: Thirty-six Kunming mice were randomly divided into normal control group, infection control group and Anluohuaxian pill group. The infected mice were challenged with 40 S. japonicum cercariae to establish a schistosomal liver fibrosis model. Intervention for 8 weeks, liver fibrosis and egg nodule changes observed; SABC immunohistochemistry αSMA and TIMP-1 expression. Results: The number of liver granuloma in Anluohuaxian Pills group was less than that in infected control group. The average diameter of granuloma of egg was smaller than that in infected control group. The levels of TIMP-1 and α SMA in liver of mice in control group Significantly decreased (P <0.05). CONCLUSION: Anluohuaxian pills can reduce the diameter of granuloma and inhibit the formation of hepatic fibrosis. Its mechanism may be to inhibit the activation of hepatic stellate cells and reduce the expression of TIMP-1 to achieve anti-fibrosis.