珠心胚(苗)是柑桔育种过程中的一个主要障碍。作者利用30年生(人工授粉时)的“Verna”柠檬与酸橙杂交获得33株实生苗,在温室中长到第三年时取成熟和健康叶片进行生化分析。结果表明:总蛋白用SDS一聚丙烯酰胺凝胶平板电泳共得到35条以上的谱带,杂种苗与珠心苗间的谱带数没有差异,仅从扫描图上发现某些带有定量(扫描峰高)差异;它们的第4条带(扫描峰)比任何一个亲本高,可能与树龄及生理年龄不 Bead embryo (seedling) is a major obstacle in citrus breeding process. The authors used 33-year-old (artificial pollination) “Verna” lemon and lime cross to obtain 33 seedlings, grown in the greenhouse to the third year of mature and healthy leaves for biochemical analysis. The results showed that more than 35 bands of total proteins were obtained by SDS-polyacrylamide gel electrophoresis. There was no difference in the number of bands between hybrid seedlings and Pearl seedlings. Only some of the bands with quantitative (Scan peak height) differences; their fourth band (scan peak) higher than any of the parents may be age and physiological age is not