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目的探讨人参皂干Rg1对NOX3在斯氏狸殖吸虫引起肝纤维化中的影响,以期发现潜在的治疗肝纤维化药物。方法 50只SD大鼠随机分为5组,每组10只。除正常组外,其他组每只大鼠腹腔注射感染斯氏狸殖吸虫囊蚴15只。8周后分别用低、中、高剂量Rg1溶液罐胃治疗该虫引起的肝纤维化,1次/d,连续8周。处死大鼠,取各组肝脏组织,用Western blot检测NOX3蛋白,qPCR检测NOX3mRNA含量;制备肝组织石蜡切片,采用免疫组化法检测肝细胞中NOX3蛋白的表达,分析Rg1对斯氏狸殖吸虫引起肝纤维化的作用。结果Western blot检测模型组大鼠NOX3含量较正常组升高(t=18.7,P<0.055,F=5.019,R square=0.9832);Rg1罐胃治疗组NOX3含量降低(P<0.01),以高剂量组降低更显著。低组(t=67.84,P<0.01,F=26.48,R square=0.9832),中组(t=55.41,P<0.01,F=4.269,R square=0.9980),高组(t=70.55,P<0.01,F=4.017,R square=0.8269)。qPCR检测模型组NOX3mRNA含量相对正常组升高(t=7.447,P<0.01,R square=0.9739),治疗组含量降低。低组(t=3.648,P<0.01,F=11.07,R square=0.9269),中组(t=5.558,P<0.01,F=4.679,R square=0.9374),高组(t=6.924,P<0.01,F=12.96,R square=0.8888)。免疫组化检测模型组NOX3高表达,肝细胞结构紊乱,可见成纤维细胞浸润。Rg1低、中、高剂量组治疗后NOX3依次降低,肝细胞形态好转。结论 NOX3在斯氏狸殖吸虫引起的肝纤维化中高表达,且在斯氏狸殖吸虫引起的肝纤维化中起重要作用。Rg1能在蛋白和mRNA水平上降低NOX3的表达,可作为潜在治疗斯氏狸殖吸虫引起的纤维化药物。
Objective To investigate the effect of ginsenoside Rg1 on liver fibrosis induced by NOX3 in the genus Schizonepeta so as to find a potential drug for the treatment of hepatic fibrosis. Methods Fifty SD rats were randomly divided into 5 groups with 10 rats in each group. In addition to the normal group, 15 rats in each group were intraperitoneally injected with metacercariae of other species. After 8 weeks, the mice were treated with low, medium and high doses of Rg1 solution to treat liver fibrosis caused by this pest, once a day for 8 weeks. The rats were sacrificed and the liver tissues of each group were taken out. The NOX3 protein was detected by Western blot and the content of NOX3 mRNA by qPCR. Paraffin sections of liver tissue were prepared and the expression of NOX3 protein in hepatocytes was detected by immunohistochemistry. Cause liver fibrosis. Results Western blot showed that the content of NOX3 in model group was higher than that in normal group (t = 18.7, P <0.055, F = 5.019, R square = 0.9832) The dose group decreased more significantly. (T = 67.84, P <0.01, F = 26.48, R square = 0.9832) in the high group (t = 55.41, P <0.01, F = 4.269, R square = 0.9980) <0.01, F = 4.017, R square = 0.8269). The content of NOX3mRNA in qPCR model group was higher than that in normal group (t = 7.447, P <0.01, R square = 0.9739). (T = 3.648, P <0.01, F = 11.07, R square = 0.9269), middle group (t = 5.558, P <0.01, F = 4.679, R square = 0.9374) <0.01, F = 12.96, R square = 0.8888). The expression of NOX3 in the model group was higher than that in the untreated group, and the structure of liver cells was disorganized. The infiltration of fibroblasts was observed. After treatment Rg1 low, medium and high dose groups of NOX3 followed by decreased liver cell morphology improved. Conclusion NOX3 is overexpressed in liver fibrosis induced by Trichoplusia niger and plays an important role in liver fibrosis induced by. Rg1 can reduce the expression of NOX3 at the protein and mRNA level, and can be used as a potential fibrotic drug for treatment of Trichoplusia.