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LBD是一类具有LOB(lateral organ boundaries)结构域的基因家族,在植物发育过程中起到非常重要的作用。采用生物信息学方法,根据拟南芥LBD基因序列鉴定了普通烟草基因组中的LBD基因,并对家族成员进行了序列特征、系统发育和表达谱分析。结果表明:普通烟草基因组中共有98个LBD基因成员,其基因结构相对简单,一般含有1~3个外显子。LBD基因家族可分成I和II两大类,两类均含有CX_2CX_6CX_3C保守结构域,但II类不含有LX_6LX_3LX_6L形成的“卷曲螺旋”二级结构,根据与拟南芥LBD蛋白构建的系统发育树则可细分成5个亚家族(Ia、Ib、Ic、Id和II)。将LBD基因与表达序列标签(EST)比对,发现36个基因有EST证据;EST、芯片数据和转录组数据分析表明:LBD基因具有不同的组织表达模式,部分基因表现出组织特异性。这些研究结果为普通烟草LBD基因家族功能的深入研究奠定了基础。
LBDs are a family of genes with lateral organ boundaries (LOBs) that play a very important role in plant development. The LBD gene of Nicotiana tabacum was identified by the bioinformatics method based on the LBD gene sequence of Arabidopsis thaliana, and the sequence characteristics, phylogeny and expression profile of the members of the family were analyzed. The results showed that there are 98 LBD genes in the genome of common tobacco, whose gene structure is relatively simple and usually contains 1 to 3 exons. The LBD gene family can be divided into two major groups, I and II, both of which contain the conserved domain of CX_2CX_6CX_3C, but the II group does not contain the “coiled-coil” secondary structure formed by LX_6LX_3LX_6L. According to phylogeny constructed with Arabidopsis LBD protein The tree can be subdivided into 5 subfamilies (Ia, Ib, Ic, Id and II). The comparison between LBD gene and expressed sequence tag (EST) showed that 36 genes had EST evidence. EST, microarray data and transcriptome data analysis indicated that LBD gene had different tissue expression patterns and some of the genes showed tissue specificity. These results lay the foundation for the further study on the function of LBD gene family in common tobacco.