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通过比较健康女性和宫颈癌患者的尿蛋白质组,发现并分析差异表达蛋白,从中筛选潜在的宫颈癌的标志物。研究对象由43名宫颈癌患者(CC)和47名健康女性(HW)组成。用超速离心法沉淀尿蛋白,再用一维凝胶电泳(SDS-PAGE)与液相色谱-质谱联用技术(LC-MS/MS)鉴定尿液中的蛋白质,蛋白质定量采用无标定量。比较患者尿蛋白质组、健康对照的尿蛋白质组和宫颈癌组织蛋白质组,有1910个蛋白质是患者和健康对照共有的尿蛋白,这其中有746个蛋白质也存在于宫颈癌组织蛋白质组。在这746个蛋白质中找到84个上调蛋白和82下调蛋白。通过生物信息学分析发现牛皮癣素(S100A7)和癌胚抗原相关细胞黏附分子8(CEACAM8)是宫颈癌尿液样本独有蛋白质。在验证组的70例样本中,双盲法测试S100A7、CEACAM8以及两者联合诊断宫颈癌的敏感性能达到73%、87%、93%。结果提示,宫颈癌患者的尿蛋白质组与健康女性的尿蛋白质组不同,并且S100A7和CEACAM8可以作为宫颈癌潜在的肿瘤标志物。
By comparing urinary proteomes in healthy women and cervical cancer patients, differentially expressed proteins were found and analyzed, and potential cervical cancer markers were screened. The study consisted of 43 cervical cancer patients (CC) and 47 healthy women (HW). Urine protein was precipitated by ultracentrifugation, and the protein in urine was identified by one-dimensional gel electrophoresis (SDS-PAGE) and liquid chromatography-mass spectrometry (LC-MS / MS). In comparison with urinary proteome of patients, urinary proteomes of healthy controls and cervical cancer proteomes, 1910 proteins are urinary proteins common to patients and healthy controls, of which 746 proteins are also present in the cervical cancer proteome. In the 746 proteins, 84 up-regulated proteins and 82 down-regulated proteins were found. Bioinformatics analysis revealed that psorigenin (S100A7) and carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8) are the unique proteins of cervical cancer urine samples. In the 70 patients in the validation group, the sensitivities of S100A7, CEACAM8 and their combination in diagnosing cervical cancer were 73%, 87% and 93%, respectively. The results suggest that the urinary proteome of cervical cancer patients is different from that of healthy women, and S100A7 and CEACAM8 may be potential tumor markers of cervical cancer.