论文部分内容阅读
为了探索小麦抗白粉病分子机理,明确茉莉酸对小麦白粉病抗性的诱导作用、对植物抗病性标志基因PR-1、PR-2、PR-5和本实验室克隆的1个新基因Ta-JA2的激活作用,以及抗病性变化与基因表达变化之间的相关性,本研究以感白粉病的小麦品种“中国春”、“濮麦9号”和“周麦18”为材料,用茉莉酸甲酯(methyl jasmonate,MeJA)喷洒小麦幼苗叶片进行诱导,通过离体叶段培养法接种白粉菌(Blumeria graminisf.sp.tritici,Bgt)进行抗性鉴定;用实时定量PCR技术检测小麦叶片中PR-1、PR-2、PR-5和Ta-JA2基因的表达变化。结果表明MeJA处理可以显著提高“中国春”、“濮麦9号”和“周麦18”对白粉菌的抗病水平。茉莉酸处理显著激活了PR-1、PR-2、PR-5和Ta-JA2的转录。茉莉酸诱导的抗病性提高与抗病标志基因PR-1、PR-2、PR-5及Ta-JA2的表达增强呈正相关。植物激素茉莉酸是小麦抗白粉病反应的信号分子。
In order to explore the molecular mechanism of wheat resistance to powdery mildew and clarify the induction of resistance to wheat powdery mildew by jasmonic acid, one new gene, PR-1, PR-2 and PR-5, Ta-JA2, and the relationship between the change of disease resistance and the gene expression. In this study, the susceptible wheat cultivars “Chinese Spring”, “Puma 9” and “Zhou” Wheat (Blumeria graminis sp. Tritici, Bgt) was inoculated by in vitro leaf culture method by spraying wheat jasmonate (MeJA) Real-time quantitative PCR was used to detect the expression of PR-1, PR-2, PR-5 and Ta-JA2 in wheat leaves. The results showed that MeJA treatment could significantly increase the disease resistance of powdery mildew of Chinese Spring, Plum 9 and Zhoumai 18. Jasmonic acid treatment significantly activated the transcription of PR-1, PR-2, PR-5 and Ta-JA2. The increase of jasmonic acid-induced disease resistance was positively correlated with the expression of disease-resistant marker genes PR-1, PR-2, PR-5 and Ta-JA2. Phytohormone jasmonic acid is a signal molecule of wheat resistance to powdery mildew.