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采用染色体特异DNA探针的荧光原位杂交(FISH)对阐明其他未查明标志染色体的起源,已证明是一种有用的补充方法。 取3例急粒和1例急淋患者骨髓和周围血细胞进行研究。细胞在含6％FCS,青霉素/链霉素(50μg/ml : 50μg/ml)和2ML-谷酰胺的RPMI 1640培养基中培养72小时。在培养的最后24小时,以甲氨喋呤阻断和胸腺嘧啶核苷或BrDU释放同步化。G-显带技术用Seabright法。杂交法按厂家介绍的方法稍加修改。杂交后的载片在45℃50％甲酰胺2×SSC
Fluorescence in situ hybridization (FISH) using chromosome-specific DNA probes has proven to be a useful complement to elucidate the origin of other unidentified markers. Three cases of acute pellet and one case of acute lymphocytic bone marrow and peripheral blood cells were studied. Cells were cultured in RPMI 1640 medium containing 6% FCS, penicillin / streptomycin (50 μg / ml: 50 μg / ml) and 2ML-glutamine for 72 hours. In the last 24 hours of culture, methotrexate was blocked and thymidine or BrDU release was synchronized. Seabright’s method for G-banding. Hybridization method introduced by the manufacturer slightly modified. After hybridization, the slides were incubated at 45 ° C for 50% formamide 2 × SSC