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目的研究染料木素(genistein,GEN)对人子宫内膜癌细胞系HEC-1B细胞体内外增殖的抑制作用,探讨其抗癌作用的机制。方法采用四甲基偶氮唑蓝比色分析(MTT)、病理组织切片HE染色等方法,分别检测体内外GEN对人子宫内膜癌细胞系HEC-1B增殖的抑制效果。结果体外实验:GEN浓度为0~25μmol/L时,对HEC-1B细胞有明显促进增殖作用(P<0.05),随着GEN浓度升高至50μmol/L时,对HEC-1B细胞则呈现抑制作用,当GEN浓度为100μmol/L时,则显示了明显的生长抑制作用(P<0.05),其抑制率达到55%。体内实验:人子宫内膜癌裸鼠模型经100μmol/L染料木素处理后体内平均瘤质量明显减小(P<0.05),抑瘤率可达39%,且处理后肿瘤组织微血管数量减少,部分区域坏死明显。结论染料木素能够明显抑制人子宫内膜癌细胞及其移植瘤的生长,其机理可能为通过和雌激素竞争结合雌激素受体,减轻雌激素促细胞增殖作用和抑制肿瘤血管生成导致肿瘤细胞坏死,从而发挥抗肿瘤作用。
Objective To study the inhibitory effect of genistein (GEN) on the proliferation of human endometrial carcinoma cell line HEC-1B in vitro and in vivo and to explore the mechanism of its anti-cancer effect. Methods The inhibitory effect of GEN on proliferation of human endometrial carcinoma cell line HEC-1B was detected by MTT assay and HE staining. Results In vitro, the proliferation of HEC-1B cells was significantly enhanced when GEN concentration was 0 ~ 25μmol / L (P <0.05), while the concentration of GEN increased to 50μmol / L, HEC-1B cells were inhibited When the concentration of GEN was 100μmol / L, the obvious growth inhibitory effect was observed (P <0.05), and the inhibition rate was 55%. In vivo experiments: The average tumor mass in human uterine endometrial carcinoma treated with 100μmol / L genistein was significantly reduced (P <0.05), the tumor inhibition rate was 39%, and the number of microvessels in the tumor tissue decreased after treatment, Some areas obvious necrosis. Conclusion Genistein can significantly inhibit the growth of human endometrial cancer cells and its xenografts. The mechanism may be that the competition of estrogen with estrogen receptors by estradiol, the promotion of estrogen-induced cell proliferation and the inhibition of tumor angiogenesis may result in tumor cells Necrosis, which play antitumor effect.