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目的探讨HBV感染者血清中PreS1与HBV-DNA、HBeAg三者间的关系,进而评估PreS1在HBV感染、复制、诊断中的作用。方法收集1750例乙型肝炎患者血清,按不同HBV-M进行分组:HBsAg(+)、HBeAg(+)、HBcAb(+)为A组,HBsAg(+)、HBeAb(+)、HBcAb(+)为B组,HBsAg(+)、HBcAb(+)为C组,HBsAg(+)为D组,采用酶联免疫法(ELISA)检测乙肝病毒PreS1与HBV血清标志物,荧光定量PCR法检测HBV-DNA。结果以HBV-DNA>5×102拷贝/mL为阳性诊断标准,不同HBV-M模式中,PreS1与HBV-DNA的检出率无显著差异(P>0.01),PreS1与HBV-DNA的总符合率高于HBeAg与HBV-DNA的符合率,在HBeAg(-)患者中仍可不同程度地检出PreS1与HBV-DNA。结论PreS1可作为HBV感染、复制的一项新指标,在乙型肝炎诊断中具有很大的应用价值。
Objective To investigate the relationship between PreS1, HBV-DNA and HBeAg in HBV infected patients and to evaluate the role of PreS1 in HBV infection, replication and diagnosis. Methods Serum samples of 1750 hepatitis B patients were collected and divided into groups according to different HBV-M groups: HBsAg (+), HBeAg (+) and HBcAb (+ In group B, HBsAg (+), HBcAb (+) were in group C and HBsAg (+) was in group D. The PreS1 and HBV serum markers of HBV were detected by enzyme-linked immunosorbent assay (ELISA) DNA. Results The positive diagnostic value of HBV-DNA> 5 × 102 copies / mL was positive. There was no significant difference in the detection rates of PreS1 and HBV-DNA among different HBV-M patterns (P> 0.01) Rate higher than the coincidence rate of HBeAg and HBV-DNA in patients with HBeAg (-) still detect PreS1 and HBV-DNA to varying degrees. Conclusion PreS1 can be used as a new indicator of HBV infection and replication, which has great value in the diagnosis of hepatitis B.