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以栽培大豆幼苗子叶节愈伤组织为材料,研究多胺氧化降解产物H2O2在大豆子叶节愈伤组织丛生芽形成中的作用。结果表明:浓度为0.5 mmol·L-1时3种外源多胺(Put,Spm和Spd)都能显著促进大豆子叶节愈伤组织分化形成丛生芽,其中Spd的作用最显著,Put和Spm作用次之,而亚精胺合酶抑制剂-环己胺(CHA)对大豆子叶节丛生芽的形成有较强的抑制作用。与此对应的是,外源多胺处理促进了子叶节外植体的二胺氧化酶(Cu AO)活性和H2O2含量水平。有趣的是,用多胺氧化酶抑制剂-氨基胍(AG)和H2O2清除剂DMTU分别处理,也可显著抑制多胺对大豆子叶节愈伤组织分化形成丛生芽的促进作用;AG和DMTU在丛生芽分化上的作用,可能与AG处理导致外植体Cu AO活性和H2O2水平的降低,以及与DMTU清除H2O2的作用有关。研究结果暗示多胺降解产物H2O2可能参与多胺对大豆子叶节愈伤组织丛生芽的诱导。
In order to study the effect of oxidative degradation product of polyamine on the formation of clump bud of soybean cotyledonary node, the seedlings of cotyledonary node of soybean seedling were planted. The results showed that three kinds of exogenous polyamines (Put, Spm and Spd) at 0.5 mmol·L-1 promoted the differentiation of soybean cotyledon calluses significantly, and the effect of Spd was the most significant. Put and Spm Followed by spermine synthase inhibitor - cyclohexylamine (CHA) on the cotyledonary node bud formation has a strong inhibitory effect. Correspondingly, exogenous polyamine treatment promoted the diamine oxidase (Cu AO) activity and H2O2 content of cotyledonary node explants. Interestingly, treatment with polyamine oxidase inhibitor aminoguanidine (AG) and H2O2 scavenger DMTU also significantly inhibited the promotion of polyamine on cotyledonary bud differentiation of soybean cotyledonary node. AG and DMTU The role of clustered bud differentiation may be related to the decrease of Cu AO activity and H2O2 level induced by AG, as well as the role of DMTU in scavenging H2O2. The results suggest that H2O2, a polyamine degradation product, may be involved in polyamine induction of clump buds in soybean cotyledonary node.